Nextseq high output v2 kit chemicals

The extracted DNA was prepared following the Illumina Nextera XT DNA Library Preparation kit. Briefly, the DNA samples were enzymatically fragmented, barcoded, and purified involving magnetic beads. Samples were then quantified using a fluorometric Qubit quantification system (Life Technologies, USA), loaded on a 2200 Tape Station instrument (Agilent Technologies, USA) and normalized to 4 nM. Sequencing was performed using an Illumina NextSeq 500 sequencer with NextSeq High Output v2 kit Chemicals 150 cycles for metagenomics data sets and using an Illumina MiSeq sequencer with MiSeq reagent kit V3 600 cycles for bacterial genomes.

The extracted DNA was prepared following the Illumina Nextera XT DNA library preparation kit. Briefly, the DNA samples were enzymatically fragmented, barcoded, and purified by using magnetic beads. Samples were then quantified using fluorometric Qubit quantification system (Life Technologies, USA), loaded on a 2200 Tape Station instrument (Agilent Technologies, USA), and normalized to 4 nM. Sequencing was performed using an Illumina NextSeq 500 sequencer with NextSeq High Output v2 kit chemicals for 150 cycles.

Trying to avoid the rind, a fixed amount of 1 g of cheese belonging to the central portion was homogenized with 9 mL of phosphate-buffered saline (PBS; pH 6.5). Subsequently, 1.5 mL of each resuspended cheese sample was subjected to bacterial DNA extraction using a DNeasy PowerFood microbial kit according to the manufacturer’s instructions (Qiagen, Germany). Then, each cheese sample’s DNA concentration and purity was investigated by employing a Picodrop microtiter Spectrophotometer (Picodrop, Hinxton, UK). The extracted DNA was prepared using the Illumina Nextera XT DNA library preparation kit. Briefly, the DNA samples were enzymatically fragmented to 550 to 650 bp using a BioRuptor machine (Diagenode, Belgium), barcoded, and purified involving the Agencourt AMPure XP DNA purification beads (Beckman Coulter Genomics GmbH, Bernried, Germany). Then, samples were quantified using the fluorometric Qubit quantification system (Life Technologies, USA), loaded on a 2200 TapeStation instrument (Agilent Technologies, USA), and normalized to 4 nM. Sequencing was performed using an Illumina NextSeq 500 sequencer with NextSeq high output v2 kit chemicals (150 cycles) (Illumina Inc., San Diego, CA 92122, USA). All sequencing data were uploaded with BioProject PRJNA865096 and SRA study SRP389312.