Huc d

Manufactured by Merck Group

Sourced in United States

HuC/D is a laboratory equipment product manufactured by Merck Group. It is designed to facilitate specific research and analysis procedures. The core function of HuC/D is to support the relevant research activities, but a detailed description of its intended use or interpretation of its capabilities is not provided.

Automatically generated - may contain errors

Lab products found in correlation

Phospho histone 3, by Merck Group (2 mentions) Acetylated tubulin, by Merck Group (2 mentions) Alexa fluor 488 conjugated anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 conjugated goat anti mouse, by Thermo Fisher Scientific (1 mentions) Axiovert 200, by Yokogawa (1 mentions) Dm5500, by Leica (1 mentions) Alexa fluor 555, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Prism 7, by GraphPad (1 mentions) Fibronectin, by Merck Group (1 mentions) Observer z1 lsm700 confocal microscope, by Zeiss (1 mentions) Zen black, by Zeiss (1 mentions) Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)

5 protocols using huc d

Whole Mount Immunostaining of Zebrafish Eyes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fixed zebrafish were used for whole mount immunostaining as previously described [57 (link)] using primary antibodies against Alcama (zn-5, 1:200; ZIRC), phospho-Histone 3 (1:500; Millipore), HuC/D (1:1000; Sigma), zpr-1 (1:1000; ZIRC). Alexa-Fluor 488- conjugated anti-mouse and anti-rabbit (1:200), Alexa-Fluor 555-conjugated goat-anti mouse (1:200), Alexa-Fluor 647-conjugated goat-anti mouse (1:200), and Texas-Red conjugated anti rabbit (1:100) secondary antibodies (Molecular Probes) were used. Nuclear staining was performed using TO-PRO-3 iodide at 1:1000 dilution for 1 hour. Eyes were imaged to produce optical sections using confocal microscopy.

Muralidharan P., Sarmah S, & Marrs J.A. (2018). Retinal Wnt signaling defect in a zebrafish fetal alcohol spectrum disorder model. PLoS ONE, 13(8), e0201659.

+ Open protocol

+ Expand

Immunodetection of HNK1 and HuC/D

Check if the same lab product or an alternative is used in the 5 most similar protocols

For post-in situ immunohistochemistry with HNK1, slides were blocked for 30 min in 5% donkey serum in PBS. Slides were incubated in 1:100 HNK1 or HuC/D (Sigma clone 15A7.1) for 4 h. Immunoreactivity was visualized a rhodamine red X conjugated mouse IgM (Jackson Immuno Research) at 1:300. See Key resource Table for antibody source (Supplementary Table S4).

Lee V.M., Hernandez S., Giang B., Chabot C., Hernandez J, & de Bellard M.E. (2020). Molecular Events Controlling Cessation of Trunk Neural Crest Migration and Onset of Differentiation. Frontiers in Cell and Developmental Biology, 8, 199.

+ Open protocol

+ Expand

Whole Mount Immunofluorescence Imaging of Zebrafish Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

Embryos were fixed at the desired time or following drug treatment in 4% PFA for 24 hr at 4°C. Whole mount immunofluorescence was performed as previously described (Venkatesan et al., 2018 (link)). Primary antibodies used were pSMAD-1/5/8 (1:100 dilution) (Cell Signal Technologies), HuC/D (1:100 dilution) (Sigma), mitfa (1:100 dilution) (Venkatesan et al., 2018 (link)). AlexaFluor-488 (Invitrogen) and AlexaFluor-555 (Invitrogen) conjugated secondary antibodies were used to detect primary antibody signaling. Nuclei were counterstained with DAPI. Following staining, animals were dissected to remove yolk sack and flat mounted laterally on slides using VectaShield mounting medium. Fluorescent images were taken using a Leica DM5500 microscope with a Leica DFC365FX camera, and a Zeiss Axiovert 200 microscope outfitted with a Yokogawa spinning disk confocal scanner. Cells and structures were counted, and data was analyzed using Microsoft Excel and GraphPad Prism 7.

Gramann A.K., Venkatesan A.M., Guerin M, & Ceol C.J. (2019). Regulation of zebrafish melanocyte development by ligand-dependent BMP signaling. eLife, 8, e50047.

+ Open protocol

+ Expand

Whole-mount Immunostaining of Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

For whole-mount immunostaining, embryos were fixed in 4% PFA at 4°C overnight. Embryos were placed in blocking solution (PBS containing 0.5% Triton X-100, 1% DMSO, 5% goat serum, 1% bovine serum albumin, and 4% sodium azide) for 2 hours at room temperature. Primary antibodies against HuC/D (Sigma, 1:1000), acetylated tubulin (Sigma, 1:500), Col2α1 (Developmental Studies Hybridoma Bank 1:100), Fibronectin (Sigma, 1:400) were diluted in blocking solutions and incubated 1 to 2 days. Alexa Fluor 488-conjugated and Alexa Fluor 555-conjugated anti-mouse or anti-rabbit secondary antibodies (Molecular Probes, Grand Island, NY, USA) were used at 1:500 dilution. Nuclear staining was performed using TO-PRO-3 iodide at a 1:1000 dilution incubated for 1 hour at room temperature. Images were acquired using a Zeiss Observer Z1 LSM 700 confocal microscope (40X 1.1 NA W or 20X 0.8 NA objectives). Regions of interest in stained embryos/larvae were imaged with several confocal optical z-sections. Zen Black (Zeiss) software was used to produce maximum intensity image projections of the z-section volumes.

Sarmah S., Hawkins M.R., Manikandan P., Farrell M, & Marrs J.A. (2022). Elf3 deficiency during zebrafish development alters extracellular matrix organization and disrupts tissue morphogenesis. PLOS ONE, 17(11), e0276255.

+ Open protocol

+ Expand

Whole-Mount Immunostaining of Zebrafish Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-mount immunostaining was performed as previously described (Clendenon, Sarmah, Shah, Liu, & Marrs, 2012 (link)) using primary antibodies against HuC/D (Sigma, 1:1000), zpr-1 (ZIRC, Eugene, OR, USA; 1:1000), zpr-3 (ZIRC, 1:500), zrf-1 (ZIRC1:1000), zn-5 (ZIRC, 1:500), acetylated tubulin (Sigma, 1:500), and phospho-histone-3 (Millipore, Bellerica, MA, USA; 1:500). Alexa Fluor 488-conjugated anti-mouse and anti-rabbit secondary antibody (Molecular Probes, Grand Island, NY, USA) was used at 1:200 dilution. Alexa Fluor 488- conjugated phalloidin (Molecular Probes) was used at a 1:100 dilution. Nuclear staining was performed using TO-PRO-3 iodide at a 1:1000 dilution incubated for 1 h.

Muralidharan P., Sarmah S, & Marrs J.A. (2014). Zebrafish retinal defects induced by ethanol exposure are rescued by retinoic acid and folic acid supplement. Alcohol (Fayetteville, N.Y.), 49(2), 149-163.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!