Dmem 6046

Alpha TC1 clone 6 (ATCC Cat. No. CRL-2934, RRID:CVCL_B036), referred to as α cells (ATCC, Manassas, VA), were cultured in DMEM 6046 (Sigma-Aldrich) supplemented with 10% (v/v) fetal bovine serum (FBS; Sigma-Aldrich), 15 mM HEPES, 0.1 mM nonessential amino acids (NEAA), 1.5% (wt/vol) sodium bicarbonate, and 2.0% (wt/vol) glucose. INS-1E (RRID:CVCL_0351), referred to as β cells (AddexBio, San Diego, CA), were cultured in RPMI-1640 (Gibco) supplemented with 5% (vol/vol) FBS, 1 mM sodium pyruvate, 10 mM HEPES, and 0.05 mM 2-mercaptoethanol. Human umbilical vein endothelial cells (HUVECs) (C2519A, used at passage 5; Lonza, Walkersville, MD), were cultured in EGM-2 (PromoCell; C-22111). All cell types were negative for mycoplasma contamination (Mycoplasma Detection Kit, #B39035; BioTool) and were cultured under a humidified atmosphere with 5% CO₂ at 37°C.

Alpha TC1 clone 6 (Cat# CRL-2934 ATCC, Manassas, United States), referred to as α cells, were cultured in DMEM 6046 (Sigma-Aldrich) supplemented with 10% (vol/vol) FBS (Sigma-Aldrich), 15 mM HEPES, 0.1 mM non-essential amino acids, 1.5% (wt/vol) sodium bicarbonate and 2.0% (wt/vol) glucose. INS-1E (AddexBio, San Diego, United States), referred to as β cells, were cultured in RPMI 1640 (Gibco) supplemented with 1 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, and 5% (vol/vol) FBS. Human umbilical vein endothelial cells (HUVECs) (C2519A, Lonza, Maryland, United States; used at passage 5) were cultivated in EGM-2 (PromoCell). All cells were cultured under a humidified atmosphere with 5% CO₂ at 37°C and negative for mycoplasma contamination (Mycoplasma Detection Kit, #B39035, BioTool).