The RNA was extracted by Trizol kit and was converted to cDNA by Reverse-transcription kit. Real-time PCR assay of the indicated genes was performed by using the FastStart DNA SYBR Green I kit (Thermo, Waltham, MA). The primers of the indicated genes were: MSH2 (positive: 5′-AAGCCCAGGATGCCATTG-3′; negative: 5′-CATTTGACACGTGAGCAAAGC-3′) and GAPDH (positive: 5′-AGGTGAAGGTCGGAGTCAAC-3′; negative: 5′-CCATGTAGTTGAGGTCAATGAAGG-3′). GAPDH gene was the internal control. The RT-PCR were determined in duplicate.
Faststart dna sybr green 1 kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FastStart DNA SYBR Green I kit is a reagent kit used for real-time quantitative PCR (qPCR) analysis. The kit contains all the necessary components for the amplification and detection of target DNA sequences, including a FastStart Taq DNA polymerase, SYBR Green I dye, and reaction buffer.
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Lab products found in correlation
2 protocols using faststart dna sybr green 1 kit
1
Quantitative Gene Expression Analysis
2
Quantification of NLRP3 Expression via RT-PCR
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The RNA was extracted from a Trizol kit and converted to cDNA by a Reverse-transcription kit. A Real-time PCR assay of the indicated genes was performed using the FastStart DNA SYBR Green I kit (Thermo, Waltham, MA, U.S.A.). The primers of the indicated genes were: NLRP3 (positive: 5′-CGTGA GTCCC ATTAA GATGG AGT-3′; negative: 5′-CCCGA CAGTG GATAT AGAAC AGA-3′) and GAPDH (positive: 5′-AGGTG AAGGT CGGAG TCAAC-3′; negative: 5′-CCATG TAGTT GAGGT CAATG AAGG-3′). The GAPDH gene was the internal control. The RT-PCR was determined in duplicate [40 (link)].
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