1525 binary hplc pump

UV data were recorded on a Hewlett-Packard 8452A diode array spectrophotometer. Optical rotation measurements were made on an AUTOPOL III automatic polarimeter. IR spectra were recorded on a Shimadzu IRAffinity-1 FT-IR spectrometer. The LC-ESIMS analyses were performed on a Shimadzu UFLC system with a quadrupole mass spectrometer using a Phenomenex Kinetex C₁₈ column (3.0 mm × 75 mm, 2.6 μm) and MeCN−H₂O (with 0.1% HCOOH) gradient solvent system. HRESIMS spectra were measured using an Agilent 6538 Ultra High Definition (UHD) Accurate-Mass Q-TOF system. NMR spectra were obtained on Varian spectrometers (500 MHz for ¹H and 125 MHz for ¹³C at the University of Oklahoma; 600 MHz for ¹H and 150 MHz for ¹³C at the University of Florida) and Bruker Avance III spectrometer equipped with a nitrogen-cooled cryoprobe (600 MHz for ¹H and 150 MHz for ¹³C at Bruker Biospin Corp.) using DMSO-d₆ and CDCl₃ (Aldrich) as solvents. Column chromatography was conducted using Sephadex LH-20. HPLC was carried out using a Waters System equipped with a 1525 binary HPLC pump coupled to a 2998 PDA detector with a Phenomenex C₁₈ column (21.2 × 250 mm or 10 × 250 mm, 5 μm particle size). Glucose-¹³C₆,d₇, sodium acetate-d₃, and D₂O were purchased from Cambridge Isotope Laboratories, Inc. All other materials were purchased from Sigma-Aldrich.