To evaluate and compare apoptotic cell death in drebrin cKO and littermate control mouse lenses, embryonic E18.5 and P1 stage tissue cryosections were subjected to in-situ Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) using an ApopTag Plus Fluorescein Kit (EMD Millipore, Burlington, MA, USA) as we described earlier.12 (link) TUNEL labeled tissue sections were counter-stained with propidium iodide. Imaging was performed using a Nikon Eclipse 90i confocal microscope in conjunction with manual counting of TUNEL positive cells.
Apoptag plus fluorescein kit
Manufactured by Merck Group
Sourced in United States
The ApopTag Plus Fluorescein Kit is a laboratory product designed to detect and quantify apoptosis, a process of programmed cell death. The kit utilizes terminal deoxynucleotidyl transferase (TdT) to label DNA strand breaks, allowing for the identification of apoptotic cells through fluorescence microscopy or flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using apoptag plus fluorescein kit
1
Apoptosis Analysis in Mouse Lenses
2
Apoptosis Analysis in Mouse Lens
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