Freestyle cho suspension cho s cells

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FreeStyle CHO-suspension (CHO-S) cells are a cell line derived from Chinese Hamster Ovary (CHO) cells that are capable of growth in suspension culture. These cells are commonly used in the production of recombinant proteins and monoclonal antibodies for therapeutic and research applications.

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Lab products found in correlation

Pcoron1000 sp vsv g tagexpression vector, by Cytiva (2 mentions) Freestyle max reagent, by Thermo Fisher Scientific (1 mentions) Pcdna3 expression vector, by Thermo Fisher Scientific (1 mentions) Hek293 cells, by Korean Cell Line Bank (1 mentions) Pcmv arms1 pk2 expression vector, by Thermo Fisher Scientific (1 mentions) Cho k1 cells, by Korean Cell Line Bank (1 mentions) Qiaprep spin plasmid kit, by Qiagen (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Pmsg elisa kit, by DRG International (1 mentions) Freestyle max transfection reagent, by Thermo Fisher Scientific (1 mentions) Plasmid maxi kit, by Qiagen (1 mentions) Pgem t easy cloning vector, by Promega (1 mentions) Htrf camp assay kit, by PerkinElmer (1 mentions) Ham s f12 medium, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Opti mem medium, by Thermo Fisher Scientific (1 mentions)

2 protocols using freestyle cho suspension cho s cells

Establishment of Stable CHO Cell Line Expressing ARMS1-PK2

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The oligonucleotides used in this study were synthesized by Genotech (Daejeon,
Korea). The cloning vector (pGEMT-T easy) was purchased from Promega (Madison,
WI, USA). The pcDNA3 expression vector, pCMV-ARMS1-PK2 expression vector,
Freestyle MAX reagent, FreeStyle CHO-suspension (CHO-S) cells and AssayComplete
medium were purchased from Invitrogen (Carlsbad, CA, USA). The PathHunter
Parental CHO-K1 cell line expressing β-arrestin 2 was
purchased from DiscoveRx (San Diego, CA, USA). The pCORON1000 SP VSV-G-tag
expression vector was purchased from Amersham Biosciences (Piscataway, NJ, USA).
The PMSG-ELISA kit was purchased from DRG International (Mountainside, NJ, USA).
Restriction enzymes and DNA ligation reagents were purchased from Takara Bio
(Shiga, Japan). Fetal bovine serum (FBS) was obtained from HyClone Laboratories
(Logan, UT, USA). The cAMP Dynamic 2 immunoassay kit was purchased from Cisbio
(Codolet, France). CHO-K1 cells and HEK 293 cells were obtained from the Korean
Cell Line Bank (KCLB, Seoul, Korea). The QIAprep-Spin plasmid kit was purchased
from Qiagen (Hilden, Germany). All other reagents were obtained from
Sigma-Aldrich (St. Louis, MO, USA).

Byambaragchaa M., Joo H.E., Kim S.G., Kim Y.J., Park G.E, & Min K.S. (2022). Signal Transduction of C-Terminal Phosphorylation Regions for Equine Luteinizing Hormone/Chorionic Gonadotropin Receptor (eLH/CGR). Development & Reproduction, 26(1), 1-12.

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Preparation and Characterization of eeIFSH

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The pGEM-T easy cloning vector was purchased from Promega (Madison, WI, USA). The pCORON1000 SP VSV-G tag expression vector was purchased from Amersham Biosciences (Piscataway, NU, USA). The pcDNA3 expression vector, FreeStyle™ MAX transfection reagent, Lipofectamine-3000, and FreeStyle CHO-suspension (CHO-S) cells were provided by Invitrogen (Carlsbad, CA, USA). Ham’s F-12 medium, and OptiMEM medium were purchased from Gibco BRL (Grand Island, NY, USA). CHO-K1 and HEK 293 cells were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). A homogeneous time-resolved fluorescence (HTRF) cAMP assay kit was purchased from Cisbio (Codolet, France). Monoclonal antibodies (5A11, 11A8, and 14F5) used in the ELISA analysis and eelFSH from CHO-S cells were produced in our lab as previously reported [41 (link)]. The horseradish peroxidase (HRP) labeling of 11A8 and 14F5 monoclonal antibodies was generously performed by Medexx Inc. (Seongnam, Korea). QIAGEN Maxi plasmid kits were purchased from Qiagen Inc. (Hilden, Germany). The glass spinner flasks and disposable flasks were provided by Corning Inc. (Corning, NY, USA). All other reagents used were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Wako Pure Chemicals (Osaka, Japan).

Byambaragchaa M., Kim J.S., Park H.K., Kim D.J., Hong S.M., Kang M.H, & Min K.S. (2020). Constitutive Activation and Inactivation of Mutations Inducing Cell Surface Loss of Receptor and Impairing of Signal Transduction of Agonist-Stimulated Eel Follicle-Stimulating Hormone Receptor. International Journal of Molecular Sciences, 21(19), 7075.

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