Posaconazole

Venetoclax, rifampin, ritonavir, cobicistat, ketoconazole, fluconazole, itraconazole voriconazole, posaconazole, isavuconazole, micafungin, caspofungin, and anidulafungin were purchased from MedChemExpress (Monmouth Junction, NJ, USA). The major itraconazole metabolites, hydroxy-itraconazole and keto-itraconazole were kindly provided by Dr. Nina Isoherranen (University of Washington, Seattle, WA, USA). [³H]Estradiol-17β-D-glucuronide (EβG; specific activity, 50.1 Ci/mmol), a positive control substrate for OATP1B, was purchased from American Radiolabeled Chemicals (Saint Louis, MO, USA). [³H]Venetoclax (specific activity, 29.3 Ci/mmol) was obtained from ViTrax (Placentia, CA, USA). PEG400 was purchased from Sigma-Aldrich (Burlington, MA, USA). Dimethyl sulfoxide (DMSO), LC-MS-grade formic acid, methanol, and acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ, USA), and 8-acetoxy-trisulfopyrene was obtained from Carbosynth Limited (Compton, Berkshire, UK). Human recombinant CYP3A4 Supersomes (456207) was purchased from Corning (Glendale, AZ, USA). NADPH RapidStart Regenerating System was purchased from SEKISUI XenoTech (Kansas City, KS, USA).