The GPC experiments for the samples studied were conducted in a chloroform solution at 35 °C and at a flow rate of 1 mL/min using a Viscotek VE 1122 (Malvern, Worcestershire, UK) pump with two Mixed C PLgel styragel columns (Agilent, Santa Clara, CA, USA) in series and a Shodex SE 61 RI detector (Showa Denko, Munich, Germany). A volume of 10 μL of a chloroform sample solution (concentration 0.5% m/V) was injected into the system. The instrument was calibrated using polystyrene standards with a low dispersity.
Ve 1122
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The VE 1122 is a laser diffraction particle size analyzer manufactured by Malvern Panalytical. The instrument measures the particle size distribution of materials by analyzing the angular variation in intensity of light scattered as a laser beam passes through a dispersed particulate sample.
Automatically generated - may contain errors
Lab products found in correlation
Mixed c plgel styragel columns, by Agilent Technologies (3 mentions)
Se 61 ri detector, by Resonac (3 mentions)
Chemstation for lc, by Agilent Technologies (1 mentions)
Poly ethylene oxide standards, by Merck Group (1 mentions)
Tda 305 triple detector array, by Malvern Panalytical (1 mentions)
Plgel olexis guard, by Agilent Technologies (1 mentions)
Pl gpc 220, by Agilent Technologies (1 mentions)
Calibration kit s m 10, by Polymer Laboratories (1 mentions)
Omnisec 5, by Malvern Panalytical (1 mentions)
6 protocols using ve 1122
1
GPC Analysis of Polymer Samples
2
Aqueous GPC Analysis of Polymers
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Aqueous GPC was conducted on a Viscotek system (Malvern) equipped with an isocratic pump Viscotek VE 1122 solvent delivery system, TDA 305 triple detector array, and three TSK Gel GMPWxL column with guard column. The system was equilibrated at 30 °C in pre-filtered water containing 0.05 M NaNO3 with the flow rate set to 1 ml min−1. Polymer solutions were prepared at a concentration of about 0.5-5 mg ml−1 and an injection volume of 200 μl was used. Data collection and analysis were performed with ChemStation for LC (Agilent) and OmniSEC v. 4,6,1,354 software (Malvern). The system was calibrated with poly(ethylene oxide) standards (Sigma) ranging from 400 to 511,000 Da (Mp).
3
Gel Permeation Chromatography for Polymer Analysis
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The molar mass and molar mass distribution of the PP samples were determined by gel permeation chromatography (GPC, Agilent Technologies PL GPC 220) apparatus equipped with two columns: Agilent PLgel Olexis guard plus 3x Olexis, 30 cm, 13 µm, at 160 °C. 1,2,4-Trichlorobenzene with anti-oxidant was employed as a solvent at a flow rate of 1.0 mL/min. The data were analyzed using polystyrene calibration.
The GPC experiments for the PHA-PP samples studied were conducted in a chloroform solution at 35 °C at a flow rate of 1 mL/min using a Viscotek VE 1122 solvent delivery system with two Mixed C PL-gel styragel columns in series and a Shodex SE 61 refractive index detector. A volume of 10 μL of sample solutions in CHCl3 (concentration 0.5% m/v) was injected into the system. Polystyrene standards with low dispersity were used to generate a calibration curve.
The GPC experiments for the PHA-PP samples studied were conducted in a chloroform solution at 35 °C at a flow rate of 1 mL/min using a Viscotek VE 1122 solvent delivery system with two Mixed C PL-gel styragel columns in series and a Shodex SE 61 refractive index detector. A volume of 10 μL of sample solutions in CHCl3 (concentration 0.5% m/v) was injected into the system. Polystyrene standards with low dispersity were used to generate a calibration curve.
4
Molar Mass Determination by GPC
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The molar mass and molar-mass dispersity of the samples were determined using gel permeation chromatography conducted in chloroform solution at 35 °C with an eluent flow rate of 1 mL/min using a Viscotek VE 1122 (Malvern, UK) pump with two Mixed C PLgel styragel columns (Agilent, Santa Clara, CA, USA) in series and a Shodex SE 61 RI detector (Showa Denko, Munich, Germany). Polystyrene standards (Calibration Kit S-M-10, Polymer Laboratories) with narrow molar-mass dispersity were used to generate a universal calibration curve. The samples were measured using OmniSEC 5.0 (Viscotek, Malvern, UK) software. The molar mass loss was calculated using the equation:
where Mw0 is the initial mass-average molar mass and Mwx is the consecutive or final average molar mass.
where Mw0 is the initial mass-average molar mass and Mwx is the consecutive or final average molar mass.
5
Characterization of 3D Fiber Matrix
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The GPC experiments for the three-dimensional fibre matrix after 1, 15, 31, 51, and 71 days of incubation were conducted in chloroform solution at 35 °C and a flow rate of 1 mL/min using a VE 1122 solvent delivery system (Viscotek, Malvern, UK) with two Mixed C PL-gel styragel columns in series and a Shodex SE 61 refractive index detector (Showa Denko, Munich, Germany). A volume of 10 μL of sample solutions in CHCl3 (concentration 0.5% m/v) were injected into the system. Polystyrene standards with low dispersity were used to generate a calibration curve.
6
Measuring PHA Molar Mass by GPC
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The number-average molar mass (Mn) and the molar mass distribution index (Mw/Mn) of the obtained PHA samples were determined by GPC experiments conducted in a chloroform solution at 35 °C and at a flow rate of 1 mL/min using a Viscotek VE 1122 (Malvern, Worcestershire, UK) pump with two Mixed C PLgel Styragel columns (Agilent, Santa Clara, CA, USA) in series, and a Shodex SE 61 RI detector (Showa Denko, Munich, Germany). A volume of 10 μL of a chloroform sample solution (concentration 0.5% m/V) was injected into the system. The instrument was calibrated using polystyrene standards with low dispersity.
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