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Sensolyte nadp nadph assay

Manufactured by AnaSpec
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The SensoLyte NADP/NADPH Assay is a colorimetric kit designed to measure the levels of NADP (nicotinamide adenine dinucleotide phosphate) and NADPH (reduced form of NADP) in biological samples. The assay provides a simple and sensitive method for quantifying these important cofactors.

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Lab products found in correlation

Gsh gssg glo, by Promega (2 mentions) Carboxy h2dcfda, by Thermo Fisher Scientific (2 mentions) Facscanto, by BD (2 mentions) Mitopy1, by Merck Group (1 mentions)

2 protocols using sensolyte nadp nadph assay

1

Cellular Oxidative Status Profiling

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Total intracellular ROS was determined by staining the cells with carboxy-H2DCFDA (Invitrogen); 2 × 105 cells were incubated with 10 mM carboxy-H2DCFDA for 30 min at 37°C. Cells were washed and analyzed by flow cytometry (BD FACSCanto; BD Biosciences, San Jose, CA, USA). Intracellular NADPH level and GSH/GSSG ratio were determined using fluorimetric SensoLyte NADP/NADPH Assay (AnaSpec, Fremont, CA, USA) and GSH/GSSG-Glo (Promega) as per manufacturer’s recommendations, respectively.
Gao W., Xu Y., Chen T., Du Z., Liu X., Hu Z., Wei D., Gao C., Zhang W, & Li Q. (2019). Targeting oxidative pentose phosphate pathway prevents recurrence in mutant Kras colorectal carcinomas. PLoS Biology, 17(8), e3000425.
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2

Quantification of Cellular Oxidative Stress

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Total intracellular Cellular ROS was determined by staining the cells with carboxy-H2DCFDA (Invitrogen) and mitochondrial ROS level was determined by using a specific mitochondrial H2O2 probe, MitoPY1 (Sigma) as previously described (Dickinson and Chang, 2008 (link); Dickinson et al., 2013 ). 2×105 cells were incubated with 10 µM carboxy-H2DCFDA or 10 µM of MitoPY1 for 30 min at 37°C. Cells were washed and analyzed by flow cytometry (BD FACSCanto). Intracellular NADPH level and GSH/GSSG ratio were determined using fluorimetric SensoLyte NADP/NADPH Assay (AnaSpec) and GSH/GSSG-Glo™ (Promega), respectively.
Jin L., Li D., Alesi G.N., Fan J., Kang H.B., Lu Z., Boggon T.J., Jin P., Yi H., Wright E.R., Duong D., Seyfried N.T., Egnatchik R., DeBerardinis R.J., Magliocca K.R., He C., Arellano M.L., Khoury H.J., Shin D.M., Khuri F.R, & Kang S. (2015). Glutamate dehydrogenase 1 signals through antioxidant glutathione peroxidase 1 to regulate redox homeostasis and tumor growth. Cancer cell, 27(2), 257-270.
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