For cell cycle analysis, the cells were harvested after transfection for 24 h. The cells were then fixed with 75% ethanol at 4°C overnight, washed with cold phosphate-buffered saline and treated with RNase I, followed by a 30-min staining with propidium iodide in the dark. Cell cycle distributors were analyzed by a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA). Cell apoptosis was detected using an Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit (Abcam, Cambridge, MA, USA) and analyzed by flow cytometry as per the manufacturer's instructions.
Annexin 5 fluorescein isothiocyanate propidium iodide apoptosis detection kit
Manufactured by Abcam
Sourced in United States
The Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit is a laboratory reagent used for the detection and analysis of apoptosis (programmed cell death) in cell samples. The kit contains Annexin V-FITC, which binds to phosphatidylserine exposed on the surface of apoptotic cells, and propidium iodide, which stains the DNA of cells with compromised cell membranes. This combination allows for the identification of early apoptotic, late apoptotic, and necrotic cells through flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
3 protocols using annexin 5 fluorescein isothiocyanate propidium iodide apoptosis detection kit
1
Cell Cycle and Apoptosis Analysis
2
Cell Apoptosis Analysis in KGN Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
KGN cells (5x104 cells per well) transfected with indicated plasmids or oligonucleotides were seeded in 6-well plates and incubated for 48 h. For cell apoptosis analysis, the Annexin V-fluorescein isothiocyanate/propidium iodide Apoptosis Detection kit (Abcam) was used according to the manufacturer's instructions. Cell apoptosis was detected using the FACScan flow cytometry system equipped with CellQuest software version 5.1 (Becton, Dickinson and Company).
3
Cell Cycle and Apoptosis Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GCs transfected with indicated oligonucleotides or plasmids were seeded into 6-well plates and cultured for 48 h. For cell cycle analysis, the cells were harvested by trypsinization, washed 3 times with PBS buffer and fixed with 75% ethanol at -20°C overnight. Then the fixed cells were incubated with 0.5 µg/ml RNase A (Thermo Fisher Scientific Inc., Wilmington, MA, USA) and 100 µg/ml propidium iodide (PI) for 30 min. For apoptosis analysis, the apoptotic cells were detected with an Annexin V-fluorescein isothiocyanate/ propidium iodide apoptosis detection kit (Abcam, Cambridge, MA, USA). Both cell cycle and apoptosis were analyzed using the FACScan flow cytometry system (Becton Dickinson, San Jose, CA, USA) equipped with CellQuest software (Becton Dickinson, Mountain View, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!