Acetic anhydride ac2o

Fmoc-protected amino acids were purchased from AAPPTEC (Louisville, KY) and EMD Biosciences (San Diego, CA). Resins were obtained from Chem-Implex (Wood Dale, IL), N-methyl-2-pyrrolidinone (NMP) from Advanced ChemTech (Louisville, KY), and 4-methylmorpholine (NMM) from Sigma-Aldrich (St. Louis, MO); all were used without further purification. Acetic anhydride (Ac₂O) was purchased from Fisher Scientific (Pittsburgh, PA). All the cancer cell lines and media were obtained from American Type Culture Collection (ATCC, Manassas, VA). PathHunter assay kit was from DiscoverX technologies (Fremont, CA). For Western blot experiment, Novex^® 4–20% tris-glycine gels and cell lysis buffer were ordered from Life Technologies (Grand Island, NY) and antibodies from Abcam, Inc. (Cambridge, MA). The antibodies for the housekeeping protein glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX).

1, 2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) pre-dissolved in chloroform was obtained from Avanti Polar Lipids (Alabaster, AL). Fmoc-Arg (Pbf)-Nova Syn TGA resin (0.23 mmol/g), 9-Fluorenylmethyl-oxycarbonyl-O-succinimide (Fmoc-OSu) and all amino acids were purchased from Novabiochem, San Diego, CA. Triisopropylsilane (TIS), anisole, hexafluro-2-propanol (HFIP), 30% ammonium hydroxide [NH₃ (aq.)], piperidine were purchased from Sigma-Aldrich, St. Louis, MO. 2,2,6,6-Tetramethylpiperidine-1-oxyl-4-carboxylic acid (TOAC) was purchased from Acros Organics, Belgium. Trifluoroacetic acid (TFA), 1-hydroxybenzo-triazole (HOBt), (2-(7-Aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU), diisopropylethylamine (DIEA), M-methyl-pyrrolidone (NMP), and dichloromethane (DCM) were purchased from Applied Biosystems, Foster City, CA. Acetic anhydride (Ac₂O) and methyl-tert-butyl ether were purchased from Fisher Scientific, Pittsburgh, PA.