After the animals were sacrificed, the femoral bones were removed and fixed in formalin-buffered (4%) PBS for 24 hours, after which the femurs were decalcified in 10% nitric acid for 3 days. The bone samples were dehydrated through a graded series of ethanol (from 70% to 100%) and diaphanized in xylene. To obtain a distinct view of the defect, the orientation and alignment of the femurs were carefully considered during paraffin embedding. Longitudinal serial sections were prepared at a thickness of 6 μm and mounted on histological slides. Hematoxylin and eosin (H&E) staining was used for general histological observations. The sections were evaluated using a Leica DMLB 80 microscope and a Leica DC300FX camera (Bannockburn, IL, USA). The images were analyzed using Qwin software (version 3 for Windows).
Dc 300fx camera
Manufactured by Leica
Sourced in United States
The Leica DC 300FX is a high-performance digital camera designed for laboratory applications. It features a high-resolution sensor and advanced optics to capture detailed and accurate images for scientific and research purposes. The camera's core function is to provide clear and reliable image capture for various laboratory tasks.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using dc 300fx camera
1
Histological Analysis of Femoral Bone
2
Scratch-Induced Neuron Regeneration Assay
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Example 5
For scratch-induced assays, 200 mm2 of NT2-N cells (n=8) were seeded on poly-D-Lysin-laminin coated cell+(Sarstedt, Germany) 12 wells plasticware, and were grown for two days in order to recover completely after trypsinisation. The medium was changed 4 h before scratching and 10 pg of neurocargo-neurovita was added per well. Individual wounds were made with an injection needle (26 G×½″, 12-4.5). At least 10 scratching were made on each individual well. Alternatively the neurocargo-neurovita was added one hour before scratching. Cells were fixed with PFA (4%) 6 days post wounding and stained with crystal violet solution. Cells are imaged using a Leica DM 5000B microscope equipped with a DC 300FX camera (×20 objective) and analysed using ImageJ 1.38X Software (Wayne Rasband, NIH, USA, http://rsb.info.nih.gov/ij/) and its plug-in NeuronJ. The average percentage of neuron in regeneration is determined from 8 experiments.
3
In Vitro Neuronal Regeneration Assay
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Example 5
For scratch-induced assays, 200 mm2 of NT2-N cells (n=8) were seeded on poly-D-Lysin-laminin coated cell+(Sarstedt, Germany) 12 wells plasticware, and were grown for two days in order to recover completely after trypsinisation. The medium was changed 4 h before scratching and 10 pg of neurocargo-neurovita was added per well. Individual wounds were made with an injection needle (26G×½″, 12-4.5). At least 10 scratching were made on each individual well. Alternatively the neurocargo-neurovita was added one hour before scratching. Cells were fixed with PFA (4%) 6 days post wounding and stained with crystal violet solution. Cells are imaged using a Leica DM 5000B microscope equipped with a DC 300FX camera (×20 objective) and analysed using ImageJ 1.38X Software (Wayne Rasband, NIH, USA, rsb.info.nih.gov/ij/) and its plug-in NeuronJ. The average percentage of neuron in regeneration is determined from 8 experiments.
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