Acetylated lysine antibody 9441

Manufactured by Cell Signaling Technology

Sourced in United States, United Kingdom

The Acetylated-Lysine Antibody (9441) is a laboratory reagent designed for the detection of acetylated lysine residues in proteins. The antibody recognizes the acetylated form of lysine, a post-translational modification that plays a role in various cellular processes.

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Lab products found in correlation

Rabbit anti mouse secondary antibody, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat secondary antibody, by Santa Cruz Biotechnology (1 mentions) Mouse anti map lc3 antibody sc 376404, by Santa Cruz Biotechnology (1 mentions) Bx 795 s1274, by Selleck Chemicals (1 mentions) Mouse interferon β elisa kit, by Cusabio (1 mentions) Goat anti rabbit secondary antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Acetylated lysine (91 citations) Acetylated-lysine antibody (24 citations) Acetylated-lysine (#9441 (4 citations)

2 protocols using acetylated lysine antibody 9441

Antibody Characterization for Innate Immunity

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The mouse anti-Map-LC3 antibody (sc-376404), the rabbit polyclonal anti-mouse AIM2 antibody (sc-137967) and the goat polyclonal antibody TMEM173 (M-12) (sc-241049) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The rabbit anti-mouse β-actin (AP0060) was obtained from Bioworld Technology (Nanjing, Jiangsu, China). The rabbit Anti-NAK/TBK-1 antibody (ab40676) and rabbit anti-NAK/TBK-1 (phosphor S172) antibody (ab109272) were obtained from Abcam (Cambridge, UK). The rabbit TMEM173 polyclonal antibody (19851-1-AP) and rabbit IRF3 polyclonal antibody (11312-1-AP) are from Protein Tech (Wuhan, Hubei, China). The phosphor-IRF3 (Ser396) (4D4G) and the Acetylated-Lysine Antibody (9441) were from Cell Signaling Technology (Boston, Mass, USA). The goat anti-rabbit secondary antibody, rabbit anti-mouse secondary antibody and donkey anti-goat secondary antibody were obtained from Santa Cruz Biotechnology, Beijing ZSGB Biotechnology and Beijing Cowin Biotechnology (Beijing, China), respectively. The BX-795 (S1274) was from Selleckchem. The Fast Protein Precipitation and Concentration Kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Reagents and apparatus used in immunoblotting assays were obtained from Bio-Rad (Hercules, CA, USA). The mouse interferon-β ELISA kit (CSB-E04945m) was from Cusabio (Wuhan, Hubei, China).

Chunfa L., Xin S., Qiang L., Sreevatsan S., Yang L., Zhao D, & Zhou X. (2017). The Central Role of IFI204 in IFN-β Release and Autophagy Activation during Mycobacterium bovis Infection. Frontiers in Cellular and Infection Microbiology, 7, 169.

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Immunoprecipitation of Acetylated Proteins

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Cells were cultured in 10 cm dishes till confluency. Cells were harvested and processed as described. The anti-PC2 antibody (D3, sc-28331) from Santa Cruz Biotechnology (Dallas, TX), GRP94 antibody (ab2791) from Abcam (Cambridge, UK), or Acetylated-Lysine antibody (#9441) from Cell Signaling (Danvers, MA) was added to each lysate and allowed to incubate overnight; 50 μL of G Sepharose 4 Fast Flow (GE Healthcare, Chicago, IL) were then added, and the mixture was incubated with gentle rocking for 4 hr at 4 °C. Beads were washed four times with lysis buffer and then centrifuged to remove the buffer. The beads were resuspended in 50 μL of Urea sample buffer containing 9M of urea, 50 mM of Tris pH 6.8, 2% SDS, 10% glycerol and 5% mercaptoethanol, vortexed for 1 min, and loaded on 3–8% Tris-Acetate or 4–12% Bis-Tris precast gels as described.

Yao Q., Outeda-Garcia P., Xu H., Walker R., Basquin D., Qian F., Cebotaru L., Watnick T.J, & Cebotaru V. (2021). Polycystin-1 dependent regulation of polycystin-2 via GRP94, a member of HSP90 family that resides in the endoplasmic reticulum. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(10), e21865.

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