Roswell park memorial institute 1640 medium

Human breast cancer cell lines T-47D, MCF7, MDA-MB-361, SK-BR-3, HCC1954, MDA-MB-468, MDA-MB-231, and BT-549 were obtained from the ATCC (Manassas, VA, USA). MCF7, MDA-MB-361, SK-BR-3, and MDA-MB-231 cells were cultured in Dulbecco modified Eagle medium (Lonza, Basel, Switzerland), and T-47D, HCC1954, MDA-MB-468, and BT-549 were maintained in Roswell Park Memorial Institute-1640 medium (Lonza); all media were supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), penicillin (100 units/mL), and streptomycin (100 mg/mL). All cells were cultured at 37 °C in a humidified atmosphere of 5% carbon dioxide.

Human MSC1 and MSC2 mesenchymal stem cell preparations were harvested after written informed consent from the bone marrow of healthy volunteers and isolated as published previously^{51 (link),52 (link)}. MSCs were cultured in Mesenchymal Stem Cell Growth Medium (Lonza, Basel, Switzerland) with added MSCGM™ Single Quots (Lonza) at 37 °C and 5% CO₂. HS68 human dermal fibroblasts were purchased from the ATCC (Manassas, USA) and were grown in Dulbecco’s Modified Eagle Medium (Biochrom, Berlin, Germany) with 10% fetal bovine serum and 3.5 g/L glucose. Human MRC5 pulmonary fibroblasts were obtained from the ATCC and were proliferated in Eagle’s Minimum Essential Medium (Sigma-Aldrich, Munich, Germany) supplemented with 10% fetal bovine serum. A549 lung carcinoma cells were received from the ATCC and grown in Roswell Park Memorial Institute-1640 medium (Lonza) including 10% fetal bovine serum. This study was approved by the independent ethics board of the University of Heidelberg (S-348/2004), and all experiments were performed according to the approved guidelines.