Sc 80663

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-80663 is a lab equipment product offered by Santa Cruz Biotechnology. It is a high-quality piece of equipment designed for use in scientific research and analysis. The core function of Sc-80663 is to provide precise and reliable measurements for various applications within the laboratory setting. Further details on the intended use or specific features of this product are not available.

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Lab products found in correlation

Sc 47778, by Santa Cruz Biotechnology (1 mentions) Ab150079, by Abcam (1 mentions) Ab217572, by Abcam (1 mentions) Ab76245, by Abcam (1 mentions) Ab150115, by Abcam (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Blocking buffer, by LI COR (1 mentions) Gadph, by Cell Signaling Technology (1 mentions) Ab150372, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) A2228, by Merck Group (1 mentions) Odyssey infrared imaging, by LI COR (1 mentions)

2 protocols using sc 80663

Immunoblotting Analysis of Protein Markers

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Proteins separated by SDS-PAGE were transferred to polyvinylidene difluoride membranes. The membranes were incubated in a solution containing mouse anti-ADAM17 antibody (sc-390859, Santa Cruz Biotechnology), mouse anti-L1CAM antibody (sc-53386, Santa Cruz Biotechnology), mouse anti-desmoglein-2 antibody (sc-80663, Santa Cruz Biotechnology), rabbit anti-galectin-3 antibody (ab76245, Abcam), rabbit anti-galectin-3 binding protein (G3BP) antibody (ab217572, Abcam), rabbit anti-clusterin antibody (42143S, Cell Signaling Technology, MA, USA), and mouse anti-beta-actin (ACTB) antibody (sc-47778, Santa Cruz Biotechnology). After washing, bound antibodies were reacted with Alexa Fluor 647-conjugated goat anti-rabbit IgG H&L (ab150079, Abcam) or Alexa Fluor 647-conjugated goat anti-mouse IgG H&L (ab150115, Abcam). The fluorescent signals were detected using an image analyzer (Typhoon 9400 Imager).

Omoteyama K., Sato T., Sato M., Tsutiya A., Arito M., Suematsu N., Kurokawa M.S, & Kato T. (2020). Identification of novel substrates of a disintegrin and metalloprotease 17 by specific labeling of surface proteins. Heliyon, 6(12), e05804.

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Western Blot Analysis of Desmosomal Proteins

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Samples for analysis were separated using by 12% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad) for Western blotting. After pre-incubation with blocking buffer (#927–60001, Li-Cor Biosciences), membranes were incubated with primary antibody overnight at 4° C followed by washing with 0.1% Tween-20 in phosphate-buffered saline 3 times and incubated with secondary antibody conjugated to Li-Cor IRDye for 1 hour and visualized using Odyssey infrared imaging (Li-Cor Biosciences). The primary antibodies used were DSG2 (ab150372, abcam; sc-80663, Santa Cruz), DSC1(sc-398590, Santa Cruz), DSC2(AF4688, R&D systems) and β-actin (A2228, Sigma). Antibodies against p44/p42 MAPK (ERK) (#9102), Phospho-p44/42 MAPK (ERK) (Thr202/Tyr204) (#9106), AKT (#9272), Phospho-Akt (Ser473) (#9271) and GADPH (#5174) were obtained from Cell Signaling Technology.

Hausrath A.C., Grüber G., Matthews B.W, & Capaldi R.A. (1999). Structural features of the γ subunit of the Escherichia coli F1 ATPase revealed by a 4.4-Å resolution map obtained by x-ray crystallography. Proceedings of the National Academy of Sciences of the United States of America, 96(24), 13697-13702.

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