Free igf1

Manufactured by Ansh Labs

Sourced in United States

Free IGF1 is a laboratory reagent that measures the concentration of free insulin-like growth factor 1 (IGF-1) in a sample. IGF-1 is a hormone that plays a role in cell growth and development. The Free IGF1 assay provides a quantitative analysis of the unbound, biologically active form of IGF-1 in a given sample.

Automatically generated - may contain errors

Lab products found in correlation

Insulin, by Merck Group (3 mentions) Leptin, by Merck Group (3 mentions) Total igf1, by Mediagnost (3 mentions) Igfbp3, by Mediagnost (3 mentions) Infinite m200, by Tecan (2 mentions) Igfbp2, by Merck Group (2 mentions)

3 protocols using free igf1

Plasma Biomarkers of Insulin Resistance

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma levels of free IGF1 (AnshLabs, Webster, TX, USA), total IGF1 (Mediagnost, Reutlingen, Germany), IGF2 (R&D Systems, Minneapolis, MN, USA), IGFBP2 (Millipore, Burlington, MA, USA), IGFBP3 (Mediagnost), insulin (Millipore) and leptin (Millipore) were measured following the manufacturers’ instructions. Absorbance was read by spectrophotometry (Tecan Infinite M200, Grödig, Austria).
Homeostatic Model Assessment for insulin Resistance (HOMA-IR) was calculated by using the following equation: HOMA-IR = glycemia (mmol/L) × insulin (mU/L)/22.5

Guerra-Cantera S., Frago L.M., Jiménez-Hernaiz M., Ros P., Freire-Regatillo A., Barrios V., Argente J, & Chowen J.A. (2020). Impact of Long-Term HFD Intake on the Peripheral and Central IGF System in Male and Female Mice. Metabolites, 10(11), 462.

+ Open protocol

+ Expand

Serum Biomarker Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum levels of free IGF1 (AnshLabs, Webster, Texas, USA), total IGF1 (Mediagnost, Reutlingen, Germany), IGF2 (BlueGene, Shangai, China), IGFBP2 (Mediagnost), IGFBP3 (Mediagnost), IGFBP5 (BlueGene), PAPP-A2 (BlueGene), insulin (Millipore, Burlington, Massachusetts, USA), and leptin (Millipore) were quantified by ELISA following the manufacturer's instructions. Non-esterified fatty-acids (NEFA) (Wako Diagnostics, Richmond, Virginia, USA) and triglycerides (Spin React, Girona, Spain) were measured by colorimetric assays as described by the manufacturers.

Guerra-Cantera S., Frago L.M., Díaz F., Ros P., Jiménez-Hernaiz M., Freire-Regatillo A., Barrios V., Argente J, & Chowen J.A. (2020). Short-Term Diet Induced Changes in the Central and Circulating IGF Systems Are Sex Specific. Frontiers in Endocrinology, 11, 513.

+ Open protocol

+ Expand

Biomarkers of Metabolic Health Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Circulating levels of free IGF1 (AnshLabs, Webster, TX, USA), total IGF1 (Mediagnost, Reutlingen, Germany), IGF2 (R&D Systems, Minneapolis, MN, USA), IGFBP2 (Millipore, Burlington, MA, USA), IGFBP3 (Mediagnost), insulin (Millipore, Burlington, MA, USA) and leptin (Millipore) were determined following the manufacturers’ instructions. Absorbance was measured by spectrophotometry (Tecan Infinite M200, Grödig, Austria). Homeostatic Model Assessment for insulin Resistance (HOMA-IR) was calculated according to the following equation:

Guerra-Cantera S., Frago L.M., Collado-Pérez R., Canelles S., Ros P., Freire-Regatillo A., Jiménez-Hernaiz M., Barrios V., Argente J, & Chowen J.A. (2021). Sex Differences in Metabolic Recuperation After Weight Loss in High Fat Diet-Induced Obese Mice. Frontiers in Endocrinology, 12, 796661.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!