Human lung bronchial epithelial cell line (BEAS-2B) and human non-small cell lung cancer cell lines (A549, NCI-H1299, NCI-H1650) were all available from ATCC (Manassas, VA, USA) and maintained at 37 °C in an incubator supplied with 5% CO2. Human non-small cell lung cancer cell line PC-9 was purchased from COBIOER Company (Nanjing, China). BEAS-2B cell line was cultivated in BEGM medium (Gibco) with LHC-9 media (Gibco, USA). F-12 K medium (Gibco) was utilized to cultivate A549 cells with 10% FBS. NCI-H1299, NCI-H1650 and PC-9 cells were routinely cultured with 10% FBS in RPMI-1640 medium (Gibco).
Begm medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
BEGM medium is a cell culture medium designed for the growth and maintenance of human bronchial epithelial cells. It provides the necessary nutrients and growth factors required for these cell types.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
F 12k medium, by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Beas 2b, by American Type Culture Collection (2 mentions)
Nci h1650, by American Type Culture Collection (1 mentions)
Lhc 9 media, by Thermo Fisher Scientific (1 mentions)
Nci h1650, by Thermo Fisher Scientific (1 mentions)
Nci h1299, by American Type Culture Collection (1 mentions)
Nci h1299, by Thermo Fisher Scientific (1 mentions)
Kyse30, by Procell (1 mentions)
Het 1a, by Thermo Fisher Scientific (1 mentions)
Het 1a cell, by American Type Culture Collection (1 mentions)
Kyse 450, by Cobioer Biosciences (1 mentions)
Hcclm3, by Thermo Fisher Scientific (1 mentions)
Sk hep1, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Beyotime (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Sk hep1, by American Type Culture Collection (1 mentions)
Thle 2, by American Type Culture Collection (1 mentions)
H1975, by Thermo Fisher Scientific (1 mentions)
4 protocols using begm medium
1
Culturing Diverse Lung Cell Lines
2
Cell Line Characterization of ESCC
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Human ESCC cell lines (ECa-109, EC9706, KYSE30 and KYSE450) and normal cell line (Het-1A) were utilized for this study and all of them were deposited with 5% CO2 at 37 °C. Het-1A cells were purchased from ATCC (Manassas, VA, USA). ECa-109 cells were purchased from Cell Bank of Chinese Academy of Sciences (Shanghai, China). EC9706 cells were purchased from Fuxiang Biotechnology Co., Ltd (Shanghai, China). KYSE30 cells were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). KYSE450 cells were purchased from Cobioer Biosciences Co., Ltd (Nanjing, China). Het-1A cells were cultured in BEGM medium (Gibco, Grant Island, NY, USA). ESCC cell lines were all cultivated in RPMI-1640 medium with 10% FBS and 1% p/s (Gibco). All cell lines have been certified using STR profiling.
3
Comparative Analysis of HCC Cell Lines
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Three HCC cell lines (HCCLM3, Huh-7 and SK-HEP-1) and one normal liver cell line (THLE-2) were utilized for this study. SK-HEP-1 and THLE-2 cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). HCCLM3 and Huh-7 were purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank. We cultured THLE-2 cells in BEGM medium (Gibco, Grand Island, NY, USA) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Beyotime, Shanghai, China), and cultured HCCLM3, Huh-7 and SK-HEP-1 cells in DMEM medium (Gibco) with the same supplements. All cells were cultured in a humidified atmosphere containing 5% CO2 at 37 °C.
4
Protocol for Establishing Murine Lung Carcinoma Models
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Female BALB/c nude mice (BALB/cAnNShjhnu) and C57BL/6 mice (C57BL/6JShjh) (6–8 weeks old) were purchased from Shanghai Jihui Laboratory Animal Care Co., Ltd (Shanghai, China) and maintained under SPF conditions in a controlled environment of 20–22 °C, with a 12/12 h light/dark cycle, 50–70% humidity, and food and water provided ad libitum. The small animal euthanasia equipment was used for animal euthanasia. Mice were put into the euthanasia chamber filling with 99.9% of CO2 gas for 10 min. All animal experiments were performed under approval by the Shanghai Medical Experimental Animal Care Commission.
All of human NSCLC cell lines (A549, H1299, HCC827, H1975, H1703, H460, and H1650), human bronchial epithelial cell line (BEAS-2B), and murine lung carcinoma cell line (LLC1) were purchased from the American Type Culture Collection (ATCC). A549 cells were cultured in F-12K medium (Gibco) with 10% FBS (Gbico). H1299, HCC827, H1975, H1703, H460, and H1650 cells were cultured in RPMI-1640 medium (Gibco) with 10% FBS. BEAS-2B cells were cultured in BEGM medium (Gibco) with 10% FBS. LLC1 cells were cultured in DMEM medium (Gibco) with 10% FBS. They were all cultured at 37 °C with 5% CO2.
All of human NSCLC cell lines (A549, H1299, HCC827, H1975, H1703, H460, and H1650), human bronchial epithelial cell line (BEAS-2B), and murine lung carcinoma cell line (LLC1) were purchased from the American Type Culture Collection (ATCC). A549 cells were cultured in F-12K medium (Gibco) with 10% FBS (Gbico). H1299, HCC827, H1975, H1703, H460, and H1650 cells were cultured in RPMI-1640 medium (Gibco) with 10% FBS. BEAS-2B cells were cultured in BEGM medium (Gibco) with 10% FBS. LLC1 cells were cultured in DMEM medium (Gibco) with 10% FBS. They were all cultured at 37 °C with 5% CO2.
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