Anti transferrin receptor tfr

Manufactured by Thermo Fisher Scientific

The Anti-Transferrin receptor (TfR) is a laboratory reagent used for the detection and quantification of the transferrin receptor (CD71) expressed on the surface of cells. The transferrin receptor is involved in the cellular uptake of iron and is expressed on actively proliferating cells. The Anti-TfR reagent can be used in various applications, such as flow cytometry, to study cellular iron metabolism and cell proliferation.

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Lab products found in correlation

P egfr, by Cell Signaling Technology (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Anti eea1, by Cell Signaling Technology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti calbindin, by Swant (1 mentions) Anti bace1 d10e5, by Cell Signaling Technology (1 mentions) Anti app 22c11, by Merck Group (1 mentions) Anti bace1, by Abcam (1 mentions) Anti gfap, by Agilent Technologies (1 mentions) Anti cd45, by BD (1 mentions)

2 protocols using anti transferrin receptor tfr

Antibody Characterization for Cell Signaling

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SNX3 antibody (rabbit, 10772–1-AP) was from Proteintech (USA). P-EGFR (rabbit, 2220) and p-AKT (rabbit, 4060) antibodies were from Cell Signaling Technology (USA). EGFR (mouse, sc-373746), AKT (rabbit, sc-8312), p-ERK 1/2 (rabbit, sc-16982), ERK 1/2 (mouse, sc-514312), CTNNB1 (mouse, sc-133240), GFP (mouse, sc-9996), and HA (mouse, MMS-101R) antibodies were from Covance (US). EGFR (rabbit, 06–847) and phosphotyrosine (pY, 4G10, mouse, 05–321) antibodies were from Millipore (Germany). Anti-Transferrin receptor (TfR) was from Thermo Fisher Scientific (13–6890). ACTB (mouse, sc-47778, Santa Cruz Biotechnology) and ACTB (mouse, A5441, Sigma-Aldrich) antibodies were used. HRP-conjugated secondary antibodies (mouse, R-05071; rabbit, R-05072) were purchased from Advansta. Fluorescent secondary antibodies (IRDye 800CW goat anti-rabbit IgG (H + L) 926–32211, IRDye 800CW goat anti-mouse IgG (H + L) 926–32210, IRDye 680LT goat anti-rabbit IgG (H + L) 926–68021, IRDye 680LT goat anti-mouse IgG (H + L) 926–68020 were purchased from Li-COR (USA). The following antibodies were used in confocal microscopy: SNX3 (Abcam, UK, ab56078), EEA1 (BD Transduction Laboratories, US 610457), Alexa 568 (Thermo Fisher Scientific, A10037), and Alexa 488 (Thermo Fisher Scientific, A21206).

Cicek E., Circir A., Oyken M., Caliskan O.A., Dioken D.N., Ergun S.G., Cetin-Atalay R., Sapmaz A., Ovaa H., Sahin O, & Erson-Bensan A.E. (2021). EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers. Oncogene, 41(2), 220-232.

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Antibody Detection of Seizure Proteins

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N-terminal antibody against seizure protein 6 (Sez6) was described previously [10 (link), 21 (link)]. For detection of seizure 6-like protein (Sez6L) we used monoclonal antibody 21D9 [19 (link)]. Additionally, immunoblotting of mouse brain tissue lysates was performed using the following antibodies: anti-BACE1 (D10E5; Cell Signaling); anti-APP (22C11; Merck Millipore) for flAPP and sAPPt; anti-sAPPβ (SIG39138; Covance) and anti-actin (Sigma Aldrich). For immunohistochemistry of mouse brain tissue, the following antibodies were used: anti-BACE1 (Epitomics; Abcam), anti-APP (Y188; Epitomics, Abcam), anti-GFAP (Dako), anti-CD45 (BD Biosciences) and anti-calbindin (Swant). Immunocytochemistry of primary mouse cortical neurons was performed using these antibodies: anti-BACE1 (Epitomics; Abcam), anti-EEA1 (Cell Signaling), anti-transferrin receptor (TfR; ThermoFisher Scientific) and anti-LAMP1 (1D4B, Santa Cruz Biotechnology).

Causevic M., Dominko K., Malnar M., Vidatic L., Cermak S., Pigoni M., Kuhn P.H., Colombo A., Havas D., Flunkert S., McDonald J., Gunnersen J.M., Hutter-Paier B., Tahirovic S., Windisch M., Krainc D., Lichtenthaler S.F, & Hecimovic S. (2018). BACE1-cleavage of Sez6 and Sez6L is elevated in Niemann-Pick type C disease mouse brains. PLoS ONE, 13(7), e0200344.

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