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Inverted phase contract microscope

Manufactured by Leica
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The Inverted Phase Contrast Microscope is a specialized laboratory instrument designed to observe and analyze transparent specimens. It features a unique optical configuration that allows for the visualization of living cells, tissues, and other translucent samples, providing detailed insights into their structure and behavior.

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Lab products found in correlation

Prism 6, by GraphPad (2 mentions)

Close spelling variants of this product

Inverted microscope (717 citations) Inverted phase microscope (6 citations) Phase-contract microscope (2 citations)

2 protocols using inverted phase contract microscope

1

Fibroblast Cell Size Imaging

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Fibroblasts were seeded at 2 × 105 cells/flask from cultures grows for two weeks with 0.25 μM MnTnBuOE-2-PyP or an equal volume of PBS for control. The following day, cells were either sham irradiated or treated with 2 Gy x-rays (Rad Source RS-2000). Cells were allowed to grow in 37°C and 5% CO2 humidified incubator for 48 hours in the presence of 0.25 μM MnTnBuOE-2-PyP or PBS, then imaged with a Leica inverted phase contract microscope. Images were imported into ImageJ and approximately 1000 cell borders were manually traced per treatment group. Areas were converted to μm2 using a conversion factor of 11.8 pixels/μm. Distributions of cell size were analyzed with GraphPad Prism 6 software (La Jolla, California).
Kosmacek E.A., Chatterjee A., Tong Q., Lin C, & Oberley R.E. (2016). MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells. Oncotarget, 7(23), 34532-34545.
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2

Prostate Fibroblast Cell Size Analysis

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Two-week-old primary prostate fibroblast cells were seeded at a concentration of 2 × 105 cells/flask in the presence or absence of 30 μM MnTE-2-PyP or an equal volume of PBS. Cells were then either sham irradiated or 2 Gy X-ray irradiated. At 48 h postirradiation, the cells were imaged using a Leica inverted phase contract microscope. Images were analyzed using ImageJ software (NIH, Bethesda, MD). Approximately 1,000 cell borders were marked per treatment group. Cell areas were converted to μm2 using a conversion factor of 11.8 pixels/μm. GraphPad Prism 6 software (GraphPad Software Inc., La Jolla, CA) was used to analyze the distributions of cell size in each group.
Chatterjee A., Kosmacek E.A, & Oberley-Deegan R.E. (2017). MnTE-2-PyP Treatment, or NOX4 Inhibition, Protects against Radiation-Induced Damage in Mouse Primary Prostate Fibroblasts by Inhibiting the TGF-Beta 1 Signaling Pathway. Radiation research, 187(3), 367-381.
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