Cultured cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with 0.3% Triton X-100. The samples were blocked with 5% BSA and incubated with specific primary antibodies overnight at 4 °C. After rinsing, the samples were incubated with fluorescence-labeled secondary antibodies at RT for 1 h. Nuclei were stained with DAPI. Slides were photographed using EVOS FL Auto 2.0 Imaging System (Thermo Fisher). Nikon Eclipse Ci-S was used for protein colocalization observation.
Evos fl auto 2.0 imaging system
Manufactured by Thermo Fisher Scientific
The EVOS FL Auto 2.0 Imaging System is a compact, automated microscope designed for high-quality digital imaging. It features a motorized stage, autofocus capabilities, and advanced imaging software to capture and analyze cell cultures and other samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using evos fl auto 2.0 imaging system
1
Immunofluorescence Staining of Cultured Cells
2
Immunofluorescence of Renal Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ACHN and 786-O cells were fixed with 4% paraformaldehyde for 15 min at room temperature, then washed with PBS 3 times and permeabilized with 0.3% Triton X-100 for 20 min at room temperature. Samples were blocked with 3% BSA (Sangon Biotech Co., Ltd.) for 30 min at room temperature and incubated with specific primary antibodies overnight at 4°C in 3% BSA. After washing, the fluorescently labelled secondary antibodies were incubated for 1 h at room temperature. After further washing, nuclei were stained with DAPI at room temperature for 10 min. Slides were observed and imaged using the EVOS FL Auto 2.0 Imaging System (Thermo Fisher Scientific, Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!