Sodium sulphate anhydrous

2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), ibuprofen, lidocaine hydrochloride, and caffeine were purchased from Sigma Aldrich (Poznań, Poland); disodium phosphate and potassium dihydrogen phosphate from Merck, Darmstadt (Germany); Biobase, beeswax were purchased from Mazidła.com (Poznań, Poland); grape seed oil was purchased from Monini (Spoleto, Italy); sodium acetate anhydrous, potassium persulfate, potassium acetate, xylene, 99.5% acetic acid, aluminium chloride, 36% hydrochloric acid, sodium chloride, potassium chloride, sodium sulphate anhydrous as well as propylene glycol, ethanol, and methanol were from Chempur (Piekary Śląskie, Poland), whereas acetonitrile for HPLC from J.T. Baker, (Landsmeer, The Netherlands). All reagents were of analytical grade.

2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyethyl cellulose (HEC), perchloric acid, 3,4-dihydroxybenzoic acid, casein, potassium persulfate, sodium linoleic salt, bovine serum, Neu reagent, diclofenac and Tris-HCl buffer were purchased from Sigma Aldrich (Steinheim am Albuch, Germany), Folin–Ciocalteu reagent, gallic acid, 4-hydroxybenzoic acid, 3-hydroxybenzoic acid, disodium phosphate, propylene glycol, and potassium dihydrogen phosphate from Merck (Darmstadt, Germany), acetic acid, aluminium chloride, hydrochloric acid, sodium sulphate anhydrous, sodium lauryl sulfate, phosphate-buffered saline (PBS) as well as propylene glycol, ethanol, methanol and isopropanol were from Chempur (Piekary Śląskie, Poland), whereas acetonitrile for HPLC from J.T. Baker, (the Netherlands). All reagents were of analytical grade.