Primary antibodies for ampkα1 α2

Manufactured by Cell Signaling Technology

Primary antibodies for AMPKα1/α2 are laboratory reagents used to detect and study the expression of the alpha subunits of the AMP-activated protein kinase (AMPK) enzyme in biological samples. AMPK is a key cellular energy sensor that plays a critical role in regulating metabolism and cellular homeostasis.

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Lab products found in correlation

Pampkα1 α2, by Cell Signaling Technology (2 mentions) Goat anti rabbit ir 800 and goat anti mouse ir 680 antibodies, by LI COR (2 mentions) Western blot gels, by Thermo Fisher Scientific (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Metformin, by Enzo Life Sciences (2 mentions) Protein sample loading buffer, by LI COR (2 mentions) Nupage lds sample buffer 4x, by Thermo Fisher Scientific (1 mentions) Nupage lds sample buffer 4, by Thermo Fisher Scientific (1 mentions) Primary antibodies for lkb1, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using primary antibodies for ampkα1 α2

Metformin Regulates AMPK and SIRT1

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Metformin was obtained from Enzo Life Sciences (Farmingdale, NY) Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for SIRT1, GAPDH and PGC1-α were obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4X Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The NuPAGE® LDS Sample Buffer (4X), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).

Matsiukevich D., Piraino G., Lahni P., Hake P.W., Wolfe V., O’Connor M., James J, & Zingarelli B. (2017). METFORMIN AMELIORATES GENDER- AND AGE-DEPENDENT HEMODYNAMIC INSTABILITY AND MYOCARDIAL INJURY IN MURINE HEMORRHAGIC SHOCK. Biochimica et biophysica acta, 1863(10 Pt B), 2680-2691.

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Metformin Regulation of AMPK Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Metformin was obtained from Enzo Life Sciences (Farmingdale, NY). Primary antibodies for AMPKα1/α2 and pAMPKα1/α2 were obtained from Cell Signaling Technology (Danvers, MA). Primary antibodies for LKB1, pLKB1 and β-actin were obtained from Santa Cruz Biotechnology (Dallas, TX). Primary antibody for PGC1-α was obtained from Abcam (Cambridge, MA). The Odyssey blocking buffer, LI-COR goat anti-rabbit IR-800 and goat anti-mouse IR-680 antibodies, and the 4× Protein Sample Loading Buffer were obtained from LI-COR Biotechnology (Lincoln, NE). The Multiplex Luminex Array kit, the NuPAGE® LDS Sample Buffer (4×), and Western blot gels were purchased from Life Technologies (Grand Island, NY). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO).

Kim P., Piraino G., O’Connor M., Hake P.W., Wolfe V., Lahni P, & Zingarelli B. (2018). Metformin exerts beneficial effects in hemorrhagic shock in an AMPKα1-independent manner. Shock (Augusta, Ga.), 49(3), 277-287.

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