Anti his monoclonal antibody

Manufactured by BioLegend

The Anti-His monoclonal antibody is a laboratory tool used for the detection and purification of proteins with a histidine (His) tag. This antibody specifically binds to the His tag, which is commonly used to facilitate the isolation and identification of recombinant proteins. The core function of this product is to enable the detection and purification of His-tagged proteins in various research applications.

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Anti-His antibody

2 protocols using anti his monoclonal antibody

Purification and Analysis of Proteins

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ATP, Mops, Tris, MgCl₂, NaCl, EDTA, glycerol, sucrose, acetic acid, lysozyme, DNAse, RNAse, Phenix imaging film, BSA, Protein G–agarose, Ni-resin and liquid scintillant were obtained from Fisher Scientific. ³²P-ATP was obtained from NEN Products. Protease inhibitor cocktail was obtained from Roche. Anti-His monoclonal antibody was purchased from BioLegend. InstantBlue was purchased from Expedeon, Hybond ECL nitrocellulose blotting membrane was purchased from Amersham and the KinaseMax™ Kit was purchased from Ambion.

Keshwani M.M., Hailey K.L., Aubol B.E., Fattet L., McGlone M.L., Jennings P.A, & Adams J.A. (2015). Nuclear Protein Kinase CLK1 Uses A Nontraditional Docking Mechanism To Select Physiological Substrates. The Biochemical journal, 472(3), 329-338.

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Purification and Characterization of Lys-C

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Adenosine triphosphate (ATP), 3-(N-morpholino)propanesulphonic acid (Mops), Tris (hydroxymethyl) aminomethane (Tris), MgCl₂, NaCl, EDTA, glycerol, sucrose, acetic acid, Lysozyme, DNAse, RNAse, Phenix imaging film, BSA, Whatman P81 grade filter paper, g-agarose, Ni-resin, and liquid scintillant were obtained from Fisher Scientific. [γ-³²P] ATP was obtained from NEN Products. Lysobacter enzymogenes endoproteinase Lys-C and protease inhibitor cocktail were obtained from Roche. Anti-His monoclonal antibody was purchased from Biolegend. InstantBlue was purchased from Expedeon, Hybond ECL nitrocellulose blotting membrane was purchased from Amersham, and the KinaseMax^™ Kit was purchased form Ambion.

Aubol B.E., Plocinik R.M., Keshwani M.M., McGlone M.L., Hagopian J.C., Ghosh G., Fu X.D, & Adams J.A. (2014). N-Terminus of the Protein Kinase CLK1 Induces SR Protein Hyper-Phosphorylation. The Biochemical journal, 462(1), 143-152.

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