HEK293 cells (ATCC® CRL-1573™) cultured in six-well plates with DMEM medium containing 10% fetal bovine serum were co-transfected with a various amounts of CMV-Shp2WT (Addgene, #8381) and ΔNp63-Flag (Addgene, #26979) at 70% confluence as indicated in Fig.5A using calcium phosphate transfection kit (Thermal-Fisher Cat: K278001) according to the manufactural protocol. 48hrs post-transfection, total protein isolated from cell lysates was subjected to western blotting to detect the expression of Shp2 and ΔNp63. To test whether Shp2 regulates ΔNp63 protein stability, HEK293 cells seeded in six-well plates were co-transfected with 1µg/well CMV-Shp2WT and 2µg/well ΔNp63-Flag and treated with a different amount of MG132 (Sigma-Aldrich, Cat:1211877–36-9) as indicated in Fig.5B . 48hrs after transfection, protein level of Shp2 and ΔNp63 was examined by western blotting analysis.
δnp63 flag
Manufactured by Merck Group
ΔNp63-Flag is a protein that functions as a transcription factor. It is used in research applications to study gene expression and cellular processes.
Automatically generated - may contain errors
2 protocols using δnp63 flag
1
Shp2 Regulates ΔNp63 Stability
2
Shp2 Regulates ΔNp63 Stability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293 cells (ATCC® CRL-1573™) cultured in six-well plates with DMEM medium containing 10% fetal bovine serum were co-transfected with a various amounts of CMV-Shp2WT (Addgene, #8381) and ΔNp63-Flag (Addgene, #26979) at 70% confluence as indicated in Fig.5A using calcium phosphate transfection kit (Thermal-Fisher Cat: K278001) according to the manufactural protocol. 48hrs post-transfection, total protein isolated from cell lysates was subjected to western blotting to detect the expression of Shp2 and ΔNp63. To test whether Shp2 regulates ΔNp63 protein stability, HEK293 cells seeded in six-well plates were co-transfected with 1µg/well CMV-Shp2WT and 2µg/well ΔNp63-Flag and treated with a different amount of MG132 (Sigma-Aldrich, Cat:1211877–36-9) as indicated in Fig.5B . 48hrs after transfection, protein level of Shp2 and ΔNp63 was examined by western blotting analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!