All chemicals and reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany) unless noted otherwise. Cell culture flasks were purchased from Greiner Bio-One (Frickenhausen, Germany). HEPES (25 mmol/L)-buffered RPMI 1640 culture medium containing 300 mg/L l -glutamine was acquired from Pan-Biotech (Aidenbach, Germany). Microvascular endothelial growth medium as per customer specification according to ATCC recommendations was acquired from Provitro AG (Berlin, Germany).
Hepes 25 mmol l buffered rpmi 1640 culture medium
Manufactured by PAN Biotech
Sourced in Germany
HEPES (25 mmol/L)-buffered RPMI 1640 culture medium is a commonly used cell culture medium that provides a buffered environment to support the growth and maintenance of various cell types. It contains the necessary nutrients, salts, and pH-buffering agents to support cell viability and proliferation in vitro.
Automatically generated - may contain errors
3 protocols using hepes 25 mmol l buffered rpmi 1640 culture medium
1
Cell Culture Methodology and Reagents
2
Cell Culture Protocol Using RPMI 1640
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All chemicals and reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany) unless noted otherwise. Cell culture flasks were purchased from Greiner Bio-One (Frickenhausen, Germany). HEPES (25 mmol/L)-buffered RPMI 1640 culture medium containing 300 mg/L L-glutamine was acquired from Pan-Biotech (Aidenbach, Germany).
3
Cell Culture Media Reagents and Suppliers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chemicals/reagents were acquired from Sigma-Aldrich (Taufkirchen, Germany) except where otherwise specified. HEPES (25 mmol/L)-buffered RPMI 1640 culture medium including 300 mg/L L glutamine was obtained from PAN-Biotech GmbH (Aidenbach, Germany) and cell culture flasks were purchased from Greiner Bio-One (Frickenhausen, Germany).
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