Escherichia coli xl10 gold

Manufactured by Thermo Fisher Scientific

Sourced in United States

Escherichia coli XL10-Gold is a competent bacterial strain commonly used in molecular biology applications. It is designed for high-efficiency transformation of plasmid DNA.

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Lab products found in correlation

P pastoris gs115, by Thermo Fisher Scientific (1 mentions) Pmd18 t, by Thermo Fisher Scientific (1 mentions)

2 protocols using escherichia coli xl10 gold

Constructing Recombinant Plasmids for Heterologous Expression in E. coli and Z. mobilis

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Escherichia coli XL10-Gold from Invitrogen (USA), was cultured in Lysogeny broth (LB, 10 g/L NaCl, 10 g/L tryptone, 5 g/L yeast extract). Medium was prepared according to the description in the Manual of Molecular Cloning [67 ]. Wild type Z. mobilis ZM4 was revived from frozen glycerol stocks in rich media with 5% glucose (RMG5: 50 g/L glucose, 10 g/L yeast extract, and 2 g/L KH₂PO₄) at 30 °C for 6–8 h without shaking. Shuttle vector pEZ15Asp includes origins of replication from both E. coli and Z. mobilis [44 (link)]. Strains and plasmids used in this study are listed in Table 3.Table 3

Strains and plasmids used in this study

Strains and plasmids	Description	Source
Strains
E. coli XL10-Gold	Ultracompetent cells (tetracycline and chloramphenicol resistant)	Invitrogen
Z. mobilis ZM4	Z. mobilis wild-type strain	Lab stock
Plasmids
pEZ15Asp	P15A_ori, Zymo_Ori, P_tet, Spe^R	[44 (link)]
pEZ15A-PlacUV5-EGFP	P15A_ori, Zymo_Ori, P_lacUV5::EGFP, Spe^R	This work
pEZ15A-PlacUV5-opmCherry	P15A_ori, Zymo_Ori, P_lacUV5::opmCherry, Spe^R	This work
pEZ-Dual	P15A_ori, Zymo_Ori, P_tet, Spe^R, P_lacUV5::opmCherry, P_tet::EGFP	This work

Yang Y., Shen W., Huang J., Li R., Xiao Y., Wei H., Chou Y.C., Zhang M., Himmel M.E., Chen S., Yi L., Ma L, & Yang S. (2019). Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era. Biotechnology for Biofuels, 12, 52.

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Recombinant Protein Expression in P. pastoris

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P. pastoris GS115, Escherichia coli XL10-gold and the vector pMD18-T were purchased from Invitrogen (USA). The expression vector pHBM905A is stored in our laboratory [33 (link)]. Minimal dextrose (MD), buffered minimal glycerol (BMGY) and buffered minimal methanol (BMMY) medium were prepared as described in the instructions of the P. pastoris expression manual from Invitrogen (USA).

Zhang X., Liu J., Yu X., Wang F., Yi L., Li Z., Liu Y, & Ma L. (2015). High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine. BMC Biotechnology, 15, 66.

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