Assay kits for alt

Zerumbone (ZER) was purchased from Sigma Aldrich (No.Z3902, St. Louis, MO, USA), dissolved in DMSO (Dimethyl sulfoxide). Bifendate (BIF) was purchased from Beijing Yuehe Pharmaceutical Factory (H11020980, Beijing, China). LPS was purchased from Beyotime Biotechnology (No.S1732, Shanghai, China). RPMI 1640 Medium was purchased from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Gibco (Gaithersburg, MA, USA). The assay kits for ALT, AST, SOD, GSH-Px, GSH, and MDA were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Bicinchoninic acid (BCA) assay kit and MTT were purchased from Solarbio (Beijing, China). Mouse IL-6 and TNF-α ELISA kits was purchased from ShangHai Haling Biological Technology Co., Ltd. (ShangHai, China). Rabbit anti-TLR4 antibody (#14358, 1:1000), rabbit anti-COX-2 antibody (#4842, 1:1000), rabbit anti-p-NF-κB p65 (Ser536) antibody (#3033, 1:1000), mouse anti-β-Actin antibody (#3700, 1:2000) were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals and reagents were purchased from local firms.

Eriodictyol (≥98%) was provided by Nanjing Guangrun Biological Products Co., Ltd. (Nanjing, China). Silibinin (≥98%) and D-galactosamine (D-GalN) were provided by Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Lipopolysaccharide (LPS) (055: B5) was bought from Sigma (Sigma, St. Louis, MO, USA). The assay kits for ALT, AST, AKP, γ-GT, CHE, TP, and ALB were supplied by Nanjing Jiancheng Bioengineering Institute (Nanjing, China), while SOD, MDA, and GSH were provided by Solarbio, (Beijing, China). The tunnel assay kit was purchased from Wuhan Servicebio, (Wuhan, China), and ELISA kits were purchased from FANKEWEI (Shanghai, China) used for measuring mice IL-6, IL-1β, and TNF-α.

Assay kits for ALT, AST, ALP, GGT, TC, TG, HDL-C, LDL-C, SOD, GSH, and MDA were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). H&E and Oil Red O were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). The TIANScrip RT Kit and Quant qPCR kit were purchased from TIANGEN Biotech (Beijing) Co., Ltd. (Beijing, China). Antibodies (Rabbit) against SREBP-1c (bs-1402R), CTP-1 (bs-2047R), and ACC (bs-2745R) were provided by Beijing Biosynthesis Biothchnology Co., Ltd. (Beijing, China). Antibodies against PPAR-α (ab8934) and horseradish peroxidase (HRP)-conjugated secondary antibodies (ab97051) were purchased from Abcam (Cambridge, UK).