6 well tissue culture treated plate

Manufactured by BD

Sourced in United States

The 6-well tissue culture-treated plates are a laboratory equipment used for cell culture and tissue engineering applications. The plates provide a controlled environment for the growth and maintenance of cells, tissues, or other biological samples. The 6-well design offers a multi-well format for parallel experiments or observations.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Goat polyclonal anti caspase 3, by Santa Cruz Biotechnology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Rabbit polyclonal anti nrf2, by Santa Cruz Biotechnology (1 mentions) Anti phospho akt, by Cell Signaling Technology (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Anti cox 2, by Cell Signaling Technology (1 mentions) Quantipro bca assay kit, by Merck Group (1 mentions) Expressplus 10 8, by GenScript (1 mentions)

Close spelling variants of this product

6-well plates (299 citations) Tissue culture plates (45 citations) Tissue-culture treated adherent 6-well plates (2 citations)

4 protocols using 6 well tissue culture treated plate

Cell Culture Proliferation Assay

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All human cell lines were used in accordance with institutional biosafety guidelines. MDA-MB-231 human breast cancer cells at low passage (less than 20 passages, ATCC #HTB-26) were maintained in DMEM supplemented with 10% FBS, and Chinese hamster ovary (CHO-K1) cells (ATCC #CCL-61) were cultured in DMEM/F12 containing 5% FBS and penicillin/streptomycin. T47D cells were maintained in RPMI-1640 with 10% FBS, in either media containing phenol red or without phenol red. For assays, cells were plated into 6-well tissue culture-treated plates (Falcon) at 2.5 × 10⁵ cells/well 24 hr prior to manipulation. Cells were transfected using Lipofectamine 2000 (Invitrogen) as described previously
[29 (link)]. To assess viable cell proliferation, cells were counted using a haemocytometer and trypan blue staining.

Linher-Melville K, & Singh G. (2014). The transcriptional responsiveness of LKB1 to STAT-mediated signaling is differentially modulated by prolactin in human breast cancer cells. BMC Cancer, 14, 415.

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Long-term BMDM-MEF Co-culture

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All co-cultures were plated in a 1:1 ratio of complete DMEM to
complete RPMI with 2% FBS (co-culture media, CCM) in 6-well tissue culture
treated plates (Falcon). Day 6–7 BMDMs and p4-p7 MEFs were plated
together without any additional supplementation of growth factors in 6-well
tissue culture plates, unless noted. For long-term co-culture, the media was
replenished every 2–3 days for the duration of the experiment by
removing 200 μL of media, and adding 400 μL of fresh CCM. The
total media volume remained relatively constant throughout the
experiment.

Zhou X., Franklin R.A., Adler M., Jacox J.B., Bailis W., Shyer J.A., Flavell R.A., Mayo A., Alon U, & Medzhitov R. (2018). Circuit design features of a stable two-cell system. Cell, 172(4), 744-757.e17.

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Focused Shock Wave Application in Cell Culture

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Isolated cells were plated (150 cells/mm² in 500 µL in 24-multiwells containing a glass coverslip) and allowed to attach for 48 h at 37 °C. Before the treatment, the glass coverslip was transferred from 24-multiwells to Falcon^® 6-well Tissue Culture-treated plate (3.5 cm of diameter and 2 cm of depth), fixed to the bottom and completely filled with culture medium.
ESWT was conducted using a Duolith SD-1 T-Top^® device (Storz Medical, Tägerwilen, Switzerland) with an electromagnetic cylindrical coil source of focused shock wave (Figure 1), using a 3D-printed support to achieve better focus, keep the probe stable, and fit the probe well to the 6-multiwells.
Briefly, each 6-mutiwell containing cells was placed in vertical alignment with focal area and was adjusted so that the central point of the focal area corresponded to the center of the bottom of the 6-multiwells. For this reason, a 3D-printed support was created for the coil source of ESWs and was kept in contact with and perfectly adherent to the shock unit with the 6-multiwell.

Pirri C., Fede C., Petrelli L., De Rose E., Biz C., Guidolin D., De Caro R, & Stecco C. (2022). Immediate Effects of Extracorporeal Shock Wave Therapy in Fascial Fibroblasts: An In Vitro Study. Biomedicines, 10(7), 1732.

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MCF7 Cell Protein Expression Analysis

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Cells were seeded (0.3 × 10⁵/well) in a 6-well tissue culture-treated plate (Falcon^®, Corning Incorporated, NY, USA) and left to adhere for 24 h. Next, MCF7 cells were exposed to compounds at the concentration of 3 µM for 24 h. Protein concentration was determined using a bicinchoninic acid assay (QuantiPro™ BCA Assay kit for 0.5–30 μg/mL protein, Sigma-Aldrich, Milan, Italy) following the manufacturer’s instructions as already reported [46 (link)].
The MCF7 cell lysates (20 μg/sample) were electrophoresed on a 4–20% SDS-PAGE Gel (ExpressPlus™ 10 × 8, GenScript Biotech Corporation, Nanjing, China) and transferred to nitrocellulose membranes. Nitrocellulose membranes were afterward blocked in 5% of non-fat milk or 5% of BSA, 10 mmol/L Tris pH 7.5, 100 mM NaCl, 0.1% Tween 20, and probed with the following primary antibodies: mouse anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA) (dilution 1:10,000), rabbit monoclonal anti-Akt, anti-phospho-Akt and anti-COX2 (all purchased by Cell Signaling Technology, MA, USA) (dilution 1:1000), goat polyclonal anti-caspase 3 (dilution 1:200), and rabbit polyclonal anti-Nrf-2 (dilution 1:750) (all purchased by Santa Cruz Biotechnology, CA, USA). Next, membranes were incubated and immunoreactive bands were detected as described previously [46 (link)].

Gallorini M., Di Valerio V., Bruno I., Carradori S., Amoroso R., Cataldi A, & Ammazzalorso A. (2023). Phenylsulfonimide PPARα Antagonists Enhance Nrf2 Activation and Promote Oxidative Stress-Induced Apoptosis/Pyroptosis in MCF7 Breast Cancer Cells. International Journal of Molecular Sciences, 24(2), 1316.

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