Anti human igκ fitc

Manufactured by Southern Biotech

Anti-human-Igk-FITC is a lab equipment product that functions as a fluorescently labeled antibody specific for the kappa light chain of human immunoglobulins. It is used for the detection and analysis of human kappa light chain-containing immunoglobulins in various applications.

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Anti-Igκ (4 citations) Anti-human Igκ (2 citations)

2 protocols using anti human igκ fitc

Isolation and Characterization of Murine Immune Cell Subsets

Cited 1 time

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Single-cell suspensions were prepared from spleen, bone marrow and thymus of 6-14 week old mice. Single-cell suspensions were stained with antibodies and analyzed using FACS Calibur with CellQuest software (BD Bioscence, San Diego, CA) or FlowJo software (Tree Star, Ashland, OR) (15 (link)). B220⁺CD43^-IgM^- small pre-B cells and CD4⁺CD8⁺ double-positive T cells were sorted by a MoFlo flow cytometer. Splenic B cells were purified using B cell isolation kits with MACS cell separation columns (Miltenyi Biotec, San Diego, CA). Generally we pooled bone marrow or splenic cells from 2-3 animals of the same genetic background for cell fractionation. Antibodies used are as follows: anti-mouse-Igκ-PE (BD Bioscience); anti-mouse-Igλ1,2,3-FITC (BD Bioscience); anti-human-Igκ-FITC (Southern Biotech, Birmingham, AL); anti-B220-PerCP-Cy5.5, anti-IgM-APC, anti-CD43-PE, anti-B220-FITC, anti–CD21-FITC, anti–CD23-APC, anti–CD4-FITC, anti–CD8a-PE, anti-B220-biotin (all from BD Bioscience); Streptavidin-APC (Southern Biotech).

Xiang Y., Park S.K, & Garrard W.T. (2014). A major deletion in the Vκ-Jκ intervening region results in hyper-elevated transcription of proximal Vκ genes and a severely restricted repertoire. Journal of immunology (Baltimore, Md. : 1950), 193(7), 3746-3754.

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Isolation and Analysis of Murine B Cells

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Bone marrow cells from femur and tibia bones were flushed out with PBS using a 25 G syringe needle. Spleens were disrupted and pulp dispersed in PBS. Erythrocytes were lysed with RBC lysis solution (Biological industries) and cells were washed. When indicated, cells were isolated on magnetic MACS columns (Miltenyi Biotech) by positive selection with either αCD19 magnetic beads or streptavidin magnetic beads and biotinylated αB220 (Miltenyi Biotech), according to the manufacturer’s instructions. Cell purity following isolation was assayed as <95% by flow cytometry (LSR II, BD Bioscience).
Cells from erythrocyte disrupted spleens and bone marrows were stained with the antibodies indicated and cellular composition was analyzed by flow cytometry (LSR II, BD Bioscience). The antibodies used in this report include anti-mouse-Igκ-PE (Southern Biotech), anti-human-Igκ-FITC (Southern Biotech), anti-IgM-APC (eBioscience), anti-B220-PerCP-Cy5.5 (Biolegend), anti-CD43-PE (Biolegend), anti-IgD-FITC (eBioscience). Flow cytometry output was analyzed using Flowing Software v2.5.0 (Turku Centre for Biotechnology).

Levin-Klein R., Kirillov A., Rosenbluh C., Cedar H, & Bergman Y. (2014). A Novel Pax5-Binding Regulatory Element in the Igκ Locus. Frontiers in Immunology, 5, 240.

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