Sc 133217

Manufactured by Santa Cruz Biotechnology

Sc-133217 is a laboratory instrument produced by Santa Cruz Biotechnology. The core function of this product is to perform analysis and quantification of biological samples. Further details about its intended use or capabilities are not available.

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Lab products found in correlation

Ab5095, by Abcam (2 mentions) Sc 899, by Santa Cruz Biotechnology (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Mouse anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Rabbit anti gst, by Santa Cruz Biotechnology (1 mentions) Mouse anti tubulin, by Merck Group (1 mentions) Rabbit anti flag, by Cell Signaling Technology (1 mentions) Ab5131, by Abcam (1 mentions) Ab70774, by Abcam (1 mentions) Ab26721, by Abcam (1 mentions) Ab76025, by Abcam (1 mentions) Ta811000, by OriGene (1 mentions) C15410003, by Diagenode (1 mentions) A8592, by Merck Group (1 mentions)

2 protocols using sc 133217

Comprehensive Antibody Characterization for Transcription Research

Cited 2 times

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The antibodies used were: rabbit anti-Rpb1 (sc-899; Santa Cruz Biotechnology), rabbit anti-Rpb1 (A304–405A & A304–405A, Bethyl Laboratories), rabbit anti-Rpb1 CTD pSer2 (ab5095, Abcam), rabbit anti-Spt5 (A300-868A, Bethyl Laboratories), mouse anti-Spt5 (sc-133217, Santa Cruz Biotechnology), rabbit anti-Spt5-pSer666 and -pThr806 (Twenty-first Century Century Biochemicals) previously described^{58 (link)}, rabbit anti-PP4R2 (A300–838A, Bethyl Laboratories), rabbit anti-PPP4C (A300–835A, Bethyl Laboratories), sheep anti-PP4R2-pThr173 (Division of Signal Transduction Therapy, University of Dundee Scotland)^{45 (link)}, rabbit phospho-PP1α (Thr320) antibody (2581S, Cell Signaling Technology), mouse anti-GFP (sc-9996, Santa Cruz Biotechnology), mouse anti-pan-PP1 (sc-7482, Santa Cruz Biotechnology), goat anti-PP1α (sc-6104, Santa Cruz Biotechnology), mouse anti-PP1β (sc-373782, Santa Cruz Biotechnology), rabbit anti-PP1γ (A300-906A, Bethyl Laboratories), goat anti-PP1γ (sc-6108, Santa Cruz Biotechnology), mouse anti-tubulin (T5168, Sigma-Aldrich), rabbit anti-GST (sc-459, Santa Cruz Biotechnology), mouse anti-FLAG^® M2 (F3165, Sigma-Aldrich), and rabbit anti-FLAG (2368, Cell Signaling).

Parua P.K., Kalan S., Benjamin B., Sansó M, & Fisher R.P. (2020). Distinct Cdk9-phosphatase switches act at the beginning and end of elongation by RNA polymerase II. Nature Communications, 11, 4338.

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Antibody Usage in Protein Analysis

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The following antibodies were used for immunoblotting and immunoprecipitation: polyclonal anti-ZC3H4 antibody, developed against a purified GST-tagged human ZC3H4 fragment (amino acid residues 1114–1315) in this study; polyclonal anti-WDR82 antibody (kindly provided by the Roeder laboratory); anti-CK2α (10992-1-AP, Proteintech); anti-CK2β (ab76025, Abcam); anti-SPT5 (A300-869A for immunoblotting; A300-868A for immunoprecipitation, Bethyl Laboratories); anti-RNAPII (ab26721, Abcam); anti-S5p RNAPII (ab5131, Abcam); anti-S2p RNAPII (ab5095, Abcam); anti-β-actin (TA811000, Origene); anti-phospho-CK2 substrate [(pS/pT)DXE] (8738, Cell Signaling Technology) and anti-FLAG (A8592, Sigma-Aldrich). The following antibodies were used for ChIP-seq analyses: anti-ZC3H4 (this study) and anti-WDR82 (48 (link)), generated in house; anti-CK2α (ab70774, Abcam); anti-SPT5 (sc-133217, Santa Cruz); anti-RNAPII (A300-653A, Bethyl Laboratories); anti-S5p RNAPII (ab5131, Abcam); anti-Sp2 RNAPII (A300-654A, Bethyl Laboratories); anti-H3K4me3 (C15410003, Diagenode); anti-H3K27me3 (9733, Cell Signaling Technology) and anti-H3K36me3 (61101, Active Motif).

Park K., Zhong J., Jang J.S., Kim J., Kim H.J., Lee J.H, & Kim J. (2022). ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. Nucleic Acids Research, 50(7), 3835-3851.

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