Acetonitrile with 0.1 formic acid

Ricin, RCA and biotinylated and non-biotinylated polyclonal goat anti-Ricinus communis antibody were obtained from Vector Laboratories (Burlingame, CA). Bovine Serum Albumin (BSA) standard reference material (SRM 927d) was obtained from the National Institute for Standards and Technology (NIST) (Gaithersburg, MD). Trypsin Gold, Mass Spectrometry Grade was purchased from Promega Corporation (Madison, WI). RapiGest SF Surfactant was purchased from Waters Corporation (Milford, MA). Dynabeads MyOne Streptavidin T1 magnetic beads were obtained from Life Technologies (Carlsbad, CA). Native heavy-labeled peptide standards were synthesized by Midwest Biotech, Inc. (Fishers, IN). The DNA substrate (5′-GCGCGAGAGCGC-3′) for measurement of N-glycosidase activity was synthesized by Integrated DNA Technologies (Coralville, IA). Adenine was purchased from Sigma–Aldrich (St. Louis, MO) and ¹³C-labeled Adenine was purchased from Moravek Biochemicals and Radiochemicals (Brea, CA). Tris (2-carboxyethyl) phosphine (TCEP) was obtained from Thermo Fisher Scientific (Rockford, IL). HPLC grade water, HPLC grade water with 0.1% formic acid, acetonitrile, acetonitrile with 0.1% formic acid and Methanol (Honeywell, Burdick and Jackson) were obtained from VWR (West Chester, PA). All other chemicals were purchased from Sigma–Aldrich (St. Louis, MO) and were used without further purification.