Cells were plated into 96-well flat clear bottom polystyrene tissue-culture treated microplates (Corning, NY, USA) and allowed to adhere for overnight. GFP-expressing 1st generation (C101), 2nd generation (C154) or the wild-type (M2001) oHSV-1 were added at indicated MOI and the plates were transferred into the IncuCyte ZOOM platform which was housed inside a cell incubator at 37 °C with 5% CO2, until the end of the assay. Four images per well from three technical replicates were taken every 3 hours for 3 days using a 10X objective lens and then analyzed using the IncuCyteTM Basic Software. Green channel acquisition time was 400 ms in addition to phase contrast.
96 well flat clear bottom polystyrene tissue culture treated microplates
Manufactured by Corning
Sourced in United States
The 96-well flat clear bottom polystyrene tissue-culture treated microplates are a type of laboratory equipment designed for cell culture applications. They provide a standardized multi-well format for performing various cell-based assays and experiments. The plates are made of polystyrene, a common material used in cell culture products, and the bottom of each well is clear to facilitate optical measurements and monitoring of cell growth.
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Lab products found in correlation
2 protocols using 96 well flat clear bottom polystyrene tissue culture treated microplates
1
Tracking Oncolytic Herpes Simplex Virus
2
Quantifying Virus-Cell Dynamics
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Cells were plated into 96-well flat clear bottom polystyrene tissue culture-treated microplates (Corning) and allowed to adhere overnight. C134 or C170 was added at the indicated MOI, and the plates were transferred into the IncuCyte ZOOM platform, which was housed inside a cell incubator at 37°C with 5% CO2, until the end of the assay. Four images per well from three technical replicates were taken every 3 hours for 3 days using a ×10 objective lens and then analyzed using the IncuCyte Basic Software. Green channel acquisition time was 400 ms in addition to phase contrast.
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