Clone do 1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Clone DO-1 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for cell culture applications, but its core function is to detect and quantify the expression of the p53 protein in cellular samples.

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Lab products found in correlation

Mouse monoclonal anti ph2ax ser 139, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg hrp, by Fortis Life Sciences (1 mentions) Goat anti rabbit igg hrp, by Fortis Life Sciences (1 mentions) Anti hsp90, by Proteintech (1 mentions) Anti hsp70, by Proteintech (1 mentions) Mouse monoclonal anti chk1, by Santa Cruz Biotechnology (1 mentions) Clone f 11, by Santa Cruz Biotechnology (1 mentions) Anti histone h3, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions) 4e bp1 rabbit polyclonal ab, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Mouse monoclonal anti-p53 (clone DO-1, (2 citations) Antibody clone DO-1 (2 citations) Anti-p53 clone DO-1 (2 citations) P53 (clone DO-1, (2 citations) Human p53 (clone DO-1, (2 citations)

2 protocols using clone do 1

Evaluating Protein Expression via Western Blot

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To evaluate protein expression on Western blot membranes the following antibodies were used: mouse monoclonal anti-pH2AX (Ser 139) (1:100) (Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-517348), mouse monoclonal anti-CHK1 (1:100) (clone F-11, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-8408), mouse monoclonal anti-RAD51 (1:100) (clone G-4, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-398587), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-126), rabbit polyclonal anti-p21 (1:200) (clone C-19, Santa Cruz Biotechnology Inc., Dallas, TX, USA, sc-397), rabbit polyclonal anti-HSP70 (1:1000) (Proteintech, Rosemont, IL, USA, 10995-1-AP) and rabbit polyclonal anti-HSP90 (1:1000) (Proteintech, Rosemont, IL, USA, 13171-1-AP). Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich, Burlington, MA, USA) or rabbit polyclonal anti-Histone H3 (4499, Cell Signaling, Danvers, MA, USA) were used as loading control. The goat anti-mouse IgG-HRP (1:30,000) (Bethyl Laboratories, Montgomery, TX, USA, A90-116P) and goat anti-rabbit IgG-HRP (1:30,000) (Bethyl Laboratories, Montgomery, TX, USA, A120-101P) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS-0.1% Tween20 solution containing 3% of BSA (SERVA).

Romeo M.A., Gilardini Montani M.S., Benedetti R., Arena A., D’Orazi G, & Cirone M. (2022). VPA and TSA Interrupt the Interplay between mutp53 and HSP70, Leading to CHK1 and RAD51 Down-Regulation and Sensitizing Pancreatic Cancer Cells to AZD2461 PARP Inhibitor. International Journal of Molecular Sciences, 23(4), 2268.

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Antibody Panel for Cellular Signaling

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The following primary antibodies were used for this study (dilutions were 1 : 1000 unless otherwise noted): 4E-BP1 Rabbit Polyclonal Ab (Cell Signaling Technology [CST], Danvers, MA, USA; Cat# 9452), Phospho-4E-BP1 (T37/46) Rabbit Monoclonal Ab, Clone 236B4 (CST; Cat# 2855), GAPDH Rabbit Monoclonal Ab, Clone 14C10 (CST; Cat# 2118), eIF2α Rabbit Polyclonal Ab (CST; Cat# 9722), eIF2α Mouse Polyclonal Ab (CST; Cat# 2103), Phospho-eIF2α (S51) XP Rabbit Monoclonal Ab, Clone D9G8 (CST; Cat# 3398), γ-Tubulin Rabbit Polyclonal Ab (Santa Cruz Biotechnology, Dallas, TX, USA; Cat# sc-7396-R), RPS27A Mouse Monoclonal Ab, Clone 3E2-E6 (Abcam, Cambridge, UK; Cat# ab57646), Cas9 Mouse Monoclonal Ab, Clone 7A9–3A3 (Active Motif, Carlsbad, CA, USA; Cat# 61578), RPS10 Rabbit Polyclonal Ab (Novus, Littleton, CO, USA; Cat# NBP1–98599), RPL10A Rabbit Polyclonal Ab (Bethyl, Montgomery, TX, USA; Cat# A305–062A), RPL22 Rabbit Polyclonal Ab (Abcam; Cat# ab77720), DNA-PKcs Rabbit Polyclonal Ab (1 : 5000 dilution; CST, Cat# 4602), Phospho-DNA-PKcs (Ser2056) Rabbit Monoclonal Ab, EPR5670 (Abcam; Cat# ab124918), P53 Mouse Monoclonal Ab, Clone DO-1 (1 : 500; Santa Cruz Biotechnology, Cat# sc-126), SBP Tag Mouse Monoclonal Ab, Clone SB19-C4 (Santa Cruz Biotechnology; Cat# sc-101595), and MDM2 Mouse Monoclonal Ab, Clone SMP14 (Santa Cruz Biotechnology; Cat# sc-965).

Riepe C., Zelin E., Frankino P.A., Meacham Z.A., Fernandez S.G., Ingolia N.T, & Corn J.E. (2022). Double stranded DNA breaks and genome editing trigger loss of ribosomal protein RPS27A. The Febs Journal, 289(11), 3101-3114.

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