Whole blood was collected into a citrate-treated tube and spun at 700 g for 15 minutes to remove cells. Supernatant was transferred to a second citrate-treated tube, spun at 1500 g for 20 minutes to remove platelets. Plasma was aliquoted and stored at −80°c. Plasma OT concentration was measured using an Enzyme Immunoassay kit (Bachem, S-1355) according to the manufacturer’s recommendation.
Enzyme immunoassay kit
Manufactured by Bachem
The Enzyme Immunoassay kit is a laboratory equipment designed for the detection and quantification of specific target analytes, such as proteins, hormones, or other biomolecules, in a sample. The kit utilizes enzyme-linked immunosorbent assay (ELISA) technology to facilitate the measurement of the target analyte through a colorimetric or fluorometric signal.
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Lab products found in correlation
3 protocols using enzyme immunoassay kit
1
Plasma Oxytocin Quantification Protocol
2
Genetic and Hematological Biomarkers in Asians
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DNA was extracted from the whole blood samples of the 57 Asian participants using a commercially available Genomic DNA Purification Kit (Promega Corporation, Fitchburg, WI). A 439 bp long segment centering on rs9366637 was PCR amplified (forward primer: 5′-ATGGTACACTGGGCTTTGGT-3′; reverse primer: 5′-TAGTGCTGAGAAAACCCGCTT-3′) and sequenced by a Sanger sequencing platform (ABI 3730xl). The genotype at rs9366637 (C/C, C/T, T/T) was visually identified from chromatograms using Chromas Lite 2.1.1 software.
Hb and hematocrit were analyzed with a hematology analyzer (Beckman Coulter, Fullerton, CA). SF was measured by a commercially available enzyme immunoassay procedure (Ramco Laboratories Inc, Stafford, TX). The concentration of sTfR was measured with an enzyme-linked immunosorbent assay (ELISA; Ramco Laboratories Inc, Stafford, TX). Serum folate, vitamin B-12, and C-reactive protein (CRP) were measured by Immulite 2000 immunoassay system (Siemens Medical Solutions Diagnostics, Los Angeles, CA). Hepcidin was determined with a commercial Enzyme Immunoassay Kit (S-1337; Bachem, San Carlos, CA).
Hb and hematocrit were analyzed with a hematology analyzer (Beckman Coulter, Fullerton, CA). SF was measured by a commercially available enzyme immunoassay procedure (Ramco Laboratories Inc, Stafford, TX). The concentration of sTfR was measured with an enzyme-linked immunosorbent assay (ELISA; Ramco Laboratories Inc, Stafford, TX). Serum folate, vitamin B-12, and C-reactive protein (CRP) were measured by Immulite 2000 immunoassay system (Siemens Medical Solutions Diagnostics, Los Angeles, CA). Hepcidin was determined with a commercial Enzyme Immunoassay Kit (S-1337; Bachem, San Carlos, CA).
3
Plasma Oxytocin Quantification Protocol
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Whole blood was collected into a citrate-treated tube and spun at 700 g for 15 minutes to remove cells. Supernatant was transferred to a second citrate-treated tube, spun at 1500 g for 20 minutes to remove platelets. Plasma was aliquoted and stored at −80°c. Plasma OT concentration was measured using an Enzyme Immunoassay kit (Bachem, S-1355) according to the manufacturer’s recommendation.
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