All reagents and solvents were obtained from commercial sources and were used as received unless otherwise stated. Phenylhydrazine hydrochloride, 4-bromophenylhydrazine hydrochloride, 4-methylphenylhydrazine hydrochloride, 4-methoxyphenylhydrazine hydrochloride, 6-bromohexanoic acid, ethyl 4-bromobutyrate, and 1-bromobutane were purchased from Meryer (Shanghai, China). 3-Methyl-2-butanone, 3-methyl-2-pentanone, 1,4-butane sultone, and isoniazid were purchased from Aladdin (Shanghai, China). Clorgyline was obtained from Bide Pharmatech Ltd. (Shanghai, China). All other reagents were synthesized in our laboratory. 1640 DMEM and 0.25% trypsin EDTA were obtained from Gibco (Sigma-Aldrich, St. Louis, MO, USA). (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). MTT reagent was acquired from Sigma-Aldrich (St. Louis, MO, USA). PC-3 and LNCaP cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China).
Dmem 1640
Manufactured by Thermo Fisher Scientific
Sourced in United States
DMEM 1640 is a cell culture medium designed for the in vitro cultivation of a variety of cell types. It provides a balanced salt solution with essential nutrients, vitamins, and amino acids to support cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
Pc 3 and lncap cells, by National Collection of Authenticated Cell Cultures (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Cck 8 assay, by Apexbio (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Ht 29 cells, by Keygen Biotech (1 mentions)
Metformin, by Beyotime (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Mrc 5, by Thermo Fisher Scientific (1 mentions)
Silicon dioxide sio2, by Merck Group (1 mentions)
Bish1475, by Sartorius (1 mentions)
Mrc 5, by Merck Group (1 mentions)
Rpmi medium 1640 basic, by Thermo Fisher Scientific (1 mentions)
Thp 1, by Merck Group (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Metformin, by Merck Group (1 mentions)
5 protocols using dmem 1640
1
Synthesis and Cytotoxicity Evaluation
2
Culturing Breast Cancer Cell Lines
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Human normal breast epithelial cells MCF-10A and BC cells MCF-7, T47D, BT549, MDAMB-231 and SKBR3 were all kept in our laboratory. BC cells were inoculated into 1640/DMEM (dulbecco's modified eagle medium) (Gibco, Rockville, MD, USA) containing 10% fetal bovine serum (FBS) (Gibco, Rockville, MD, USA) and cultured in an incubator with 5% CO 2 at 37°C. Upon reaching 80-90% confluence, the cells were passaged.
3
Cytotoxic Effects of Phytochemicals on HT-29 Cells
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HT-29 cells were purchased from Jiangsu KeyGEN BioTECH Corp. (Jiangsu, China). The cells were cultured at 37°C and 5% CO2 in DMEM 1640 (Gibco) that contained 10% fetal bovine serum (FBS, Gibco) and 1% penicillin–streptomycin (Gibco). When the cells reached 80% confluence, they were divided and sub-cultured for further passages with 0.25% trypsin and 0.02% ethylene-diamine-tetra-acetic acid (EDTA, Gibco). Cell viability was determined using the cell counting kit-8 (CCK-8) assay (APExBIO Technology LLC, United States). Briefly, cells were seeded and treated with different concentrations of luteolin (LUT, Aladdin, Figure 2A ), β-sitosterol (SIT, Aladdin, Figure 2B ), myristic acid (MYA, J&K Scientific, Figure 2C ), and vanillin (VAN, J&K Scientific, Figure 2D ) for 24 h, and 10% CCK-8 solution was added to each well. After incubation for 2 h at 37°C, the absorbance at 490 nm was read on a microplate reader (iMark, Bio-Rad, United States).
4
Investigating Metformin's Modulation of Cell Responses to Silica Nanoparticles
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The lung epithelial cells (A549 and HBE), lung fibroblast (MRC-5), and the human monocytic cell (THP-1) were commercially obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). A549 and THP-1 cells were maintained in RPMI Medium 1640 basic (1640, Life Technologies/Gibco, Grand Island, NY, USA), the HBE cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, 1640, Life Technologies/Gibco, Grand Island, NY, USA), and the MRC-5 cells were maintained in Minimum Essential Medium (MEM, Life Technologies/Gibco, Grand Island, NY, USA). All of the culture media were containing 10% fetal bovine serum (BISH1475, Biological Industries) and antibiotics (penicillin and streptomycin, Life Technologies/Gibco, Gaithersburg, MD). Cells were cultured at 37 °C in a 5% CO2 atmosphere.
PMA (Sigma–Aldrich) was used to treat THP-1 cells into macrophages. For all the experiments analysis, epithelial cells and THP-1 were treated with 200 or 150 μg/ml Silicon dioxide (SiO2) (Sigma-Aldrich, St. Louis, MO, USA) together with various concentrations (2, 5, 10 mM) of metformin (Beyotime Institute of Biotechnology, Shanghai, China); MRC-5 cells were treated with 5 ng/ml TGF-β1 (Sigma-Aldrich) together with metformin.
PMA (Sigma–Aldrich) was used to treat THP-1 cells into macrophages. For all the experiments analysis, epithelial cells and THP-1 were treated with 200 or 150 μg/ml Silicon dioxide (SiO2) (Sigma-Aldrich, St. Louis, MO, USA) together with various concentrations (2, 5, 10 mM) of metformin (Beyotime Institute of Biotechnology, Shanghai, China); MRC-5 cells were treated with 5 ng/ml TGF-β1 (Sigma-Aldrich) together with metformin.
5
Single-cell ChIP-seq of MDA-MB-468 cells
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MDA-MB-468 cells, bought at ATCC (HTB-132™), were cultured in DMEM 1640 (Gibco-BRL) and supplemented with 10% heat-inactivated fetal calf serum. Cell numbers, as judged by Trypan Blue exclusion test, were determined by counting cells using a Countess automated cell counter (Invitrogen). Cells were cultured at 37 °C in a humidified 5% CO2 atmosphere. The cell line was mycoplasma negative. The MDA-MB-468 cells were Trypsinized (Trypsin, Gibco-BRL). Prior to single-cell ChIP-seq, cells were then re-suspended in PBS/0.04% BSA (ThermoFisher Scientific, # AM2616).
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