Acta2 specific sirna sc 43591

Manufactured by Santa Cruz Biotechnology

Sourced in United States

ACTA2-specific siRNA (sc-43591) is a small interfering RNA (siRNA) designed to target the ACTA2 gene, which encodes the alpha-2 actin protein. This product is intended for use in gene silencing experiments to study the function of the ACTA2 gene.

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Lab products found in correlation

Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (2 mentions) Specific sirna, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SiRNAs (95 citations) Specific siRNAs (18 citations)

2 protocols using acta2 specific sirna sc 43591

Silencing ACTA2 in Neural Stem Cells

Cited 1 time

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ACTA2-specific siRNA (sc-43591) was purchased from Santa Cruz Biotechnology (CA, USA). ACTA2-specific siRNA was transfected into NSC using a Lipofectamine™ 3000 transfection reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer's instructions. Meanwhile, the same volume of scramble siRNA and Lipofectamine™ 3000 transfection reagent was loaded as negative control. The transfection efficiency was determined by immunoblotting, as previously described (data not shown) [16 (link)].

Zhang J., Hu Q., Jiang X., Wang S., Zhou X., Lu Y., Huang X., Duan H., Zhang T., Ge H, & Yu A. (2022). Actin Alpha 2 Downregulation Inhibits Neural Stem Cell Proliferation and Differentiation into Neurons through Canonical Wnt/β-Catenin Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2022, 7486726.

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Silencing ACTA2 in Neural Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

ACTA2-specific siRNA (sc-43591) was purchased from Santa Cruz Biotechnology (CA, USA). ACTA2-specific siRNA were transfected into NSCs using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, Waltham, MA, USA) according to the manufacturer's instructions. The same amount of scramble siRNA and Lipofectamine™ 3000 Transfection Reagent was served as the negative control. The transfection efficiency was determined by RT-PCR and western blot.

Zhang J., Jiang X., Zhang C., Zhong J., Fang X., Li H., Xie F., Huang X., Zhang X., Hu Q., Ge H, & Yu A. (2020). Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation. Stem Cells International, 2020, 4764012.

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