Dspe peg 2000

Manufactured by Nanocs

DSPE‐PEG(2000) is a lipid-based molecule that consists of distearoylphosphatidylethanolamine (DSPE) conjugated to polyethylene glycol (PEG) with a molecular weight of 2000 Daltons. This product is commonly used as a surfactant and stabilizer in the formulation of various drug delivery systems and nanoparticles.

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Lab products found in correlation

Dspe peg 2000 maleimide, by Avanti Polar Lipids (1 mentions) Track etch membrane, by Cytiva (1 mentions)

Close spelling variants of this product

DSPE-PEG-2000-SH DSPE-PEG(2000)-Folic acid DSPE-PEG

2 protocols using dspe peg 2000

Peptide-Based Immunotherapeutic Protocol

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LTX‐315 (sequence: K‐K‐W‐W‐K‐K‐W‐Dip‐K‐NH₂), FITC‐LTX‐315 (N‐ter modification), and Cyclo(‐RGDfC) peptides were synthesized and provided by GeneCust (Rue de Chalmont, Boynes, France). Murine TGF‐β1 siRNA (Forward, GCAACAACGCCAUCUAUGA; Reverse, UCAUAGAUGGCGUUGUUGC) and Cyanine 5.5 conjugated TGF‐β1 siRNA were synthesized and provided by Bioneer (Daedeok‐gu, Daejeon, South Korea). Poly(d,l‐lactide‐co‐glycolide) (Resomer® RG 504, Mw 38,000–54,000) was purchased from Sigma Aldrich (St. Louis, MO). DSPE‐PEG(2000) was procured from Nanocs (Fifth Avenue, NY). DSPE‐PEG(2000) Maleimide was obtained from Avanti Polar Lipids (Industrial Park Drive Alabaster, Alabama). InVivoMAb anti‐mouse NKG2A/C/E antibody was received from BioXCell (West Lebanon, NH). All fluorophore‐modified antibodies used in flow cytometry were purchased from Biolegend (San Diego, CA).

Phung C.D., Nguyen B.L., Jeong J., Chang J., Jin S.G., Choi H., Ku S.K, & Kim J.O. (2022). Shaping the “hot” immunogenic tumor microenvironment by nanoparticles co‐delivering oncolytic peptide and TGF‐β1 siRNA for boosting checkpoint blockade therapy. Bioengineering & Translational Medicine, 8(5), e10392.

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Membrane-Coated Polymer Nanoparticle Synthesis

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Cell plasma membrane mixed with 300 μg of DSPE-PEG2000 (Nanocs, catalog no. 121109) were physically extruded through a 200-nm Track-Etch membrane (Whatman, catalog no. 800281) for ten passes. PPDCNPs were coated in the membrane vesicles by an extruder set with holder block (Avanti, catalog no. 610000) through a 200-nm Track-Etch membrane to form m-PPDCNPs.

Zhu S., Li Z., Zheng D., Yu Y., Xiang J., Ma X., Xu D., Qiu J., Yang Z., Wang Z., Li J., Sun H., Chen W., Meng X., Lu Y, & Ren Q. (2022). A cancer cell membrane coated, doxorubicin and microRNA co-encapsulated nanoplatform for colorectal cancer theranostics. Molecular Therapy Oncolytics, 28, 182-196.

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