Lentivirus gv358 tagln

lentivirus/GV248‐siTAGLN (hU6‐MCS‐Ubiquitin‐EGFP‐IRES‐puromycin) and lentivirus/GV358 ‐TAGLN (Ubi‐MCS‐3FLAG‐SV40‐EGFP‐IRES‐puromycin) and their corresponding control lentiviruses, lentivirus/GV248 and lentivirus/GV358 respectively, were obtained from Genechem (Shanghai, China). The recombined lentiviral particles were diluted with complete SMCM. Polybrene was added to enhance the infection efficiency. About 3.5 × 10⁴‐4.5 × 10⁴ hPASMC per well were seeded in a six‐well plate. After cell culture for 24 hours, the medium was replaced by 0.5 ml of the infection solution (LV‐GV248, LV‐siTAGLN, LV‐GV358, LV‐TAGLN) per well. 12 hours later, the infection solution was replaced by complete SMCM. The hPASMC were harvested 84 hours after infection for cell morphological observation, mRNA and protein expression, cell proliferation, apoptosis and migration. The transduction efficiency was greater than 85% as measured by the green fluorescent protein.