COV434 cell slides were prepared and probed with antibodies specific for JAK1 (ab47435, Abcam), pJAK1 (ab138005, Abcam), STAT1 (ab2415, Abcam) and STAT3 (79D7, Cell Signalling Technologies). Primary antibodies were visualised using either a goat-anti-rabbit Alexa 555 secondary antibody (ab150078, Life Technologies) or a goat-anti-mouse Alexa 555 secondary antibody (a21422, Life Technologies) at a 1:100 dilution and 4′-6-Diamidino-2-phenylindole (DAPI) was used as a nuclear counterstain. Slides were imaged using the Axio Imager A1 fluorescent microscope (Carl Zeiss MicroImaging, Inc, Thornwood, NY) and Olympus DP70 microscope camera (Olympus America, Center Valley, PA).
Dp70 microscope camera
Manufactured by Olympus
Sourced in Japan
The DP70 is a digital microscope camera produced by Olympus. It is designed to capture high-quality images from microscopes. The DP70 features a 12.5-megapixel CCD sensor and can capture images at resolutions up to 4,140 x 3,096 pixels.
Automatically generated - may contain errors
Lab products found in correlation
Cellsens software, by Olympus (2 mentions)
A21422, by Thermo Fisher Scientific (1 mentions)
Axio imager a1 fluorescent microscope, by Zeiss (1 mentions)
Ab2415, by Abcam (1 mentions)
Ab47435, by Abcam (1 mentions)
Ab138005, by Abcam (1 mentions)
Hoechst 33342 stain, by Merck Group (1 mentions)
Bx41 microscope, by Olympus (1 mentions)
6 protocols using dp70 microscope camera
1
Immunofluorescence Analysis of JAK-STAT Pathway
2
Intracellular Localization of Transcription Factors
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In order to determine the intracellular localization of the transcription factors, protein-transduced cells were examined by fluorescent microscopy. Following 24 and 72 h of transduction, cells were harvested and washed twice (5 min/wash) with phosphate-buffered saline (PBS), then fixed in 4% (v/v) formaldehyde in PBS at room temperature for 1 min. Cells were washed twice (5 min/wash) with PBS and incubated with primary anti-His tag antibody (catalogue. no. 05-949; 1:1,000; Novagen; Merck KGaA), at room temperature for 1 h. Following washing three times with PBS, fluorescence conjugated secondary anti-mouse immunoglobulin G (H+L) Alexa Fluor 488 Conjugate (catalogue no. 4408S; 1:1,000; CST Biological Reagents Co., Ltd., Shanghai, China) was added and the cells were incubated in the dark for 1 h at room temperature. Cells were subsequently washed with PBS and nuclear DNA was labeled with Hoechst 33342 stain (1:3,000; Sigma-Aldrich; Merck KGaA). Images were captured using an Olympus DP70 microscope camera (Olympus Corp., Tokyo, Japan).
3
Quantifying Uterine Pathology in Infected Mice
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Mouse uteri were excised and immersion fixed in 10% buffered neutral formalin on days 23 and 64 post-infection for scoring inflammatory damage. Following fixation, they were processed in whole and embedded en bloc into paraffin followed by sections cut at 4 μm and stained with hematoxylin and eosin (H&E). Sections were examined and scored via light microscopy by an American College of Veterinary Pathologists (ACVP) certified veterinary pathologist with the following numerical designations: 0, normal; 1, minimal change; 2, mild change; 3, moderate change; 4, severe change. Scored parameters (mean ± SE) included an overall impression, periglandular mucinous change, hydrosalpinx, uterine tubal dilation, and luminal PMN. Morphometric analyses of endometrial luminal epithelial height were determined using calibrated measurements via Olympus CellSens software coupled to an Olympus DP70 microscope camera. Each reproductive tract was evaluated regionally and morphometrically at six locations (proximal, mid-, and distal locations of the two uterine horns) determined to be uniform and free of tissue bends and curves.
4
Histological Evaluation of Mouse Uteri
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Mouse uteri were excised and immersion fixed in 10% buffered neutral formalin. Following fixation, they were processed entire and embedded en bloc into paraffin followed by sections cut at 4 μm and staining with hematoxylin and eosin (H&E). Sections were examined and scored via light microscopy by an American College of Veterinary Pathologists (ACVP) certified veterinary pathologist with the following numerical designations: 0, normal; 1, minimal change; 2, mild change; 3, moderate change; 4, severe change. Scored parameters (mean ± SD) included an overall impression, periglandular mucinous change, hydrosalpinx, uterine luminal, and stratum compactum inflammation. Additionally, endometrial luminal epithelial height was subjected to morphometric analyses focused on epithelial height as determined by calibrated measurements via Olympus CellSens software coupled to an Olympus DP70 microscope camera. Each reproductive tract was evaluated regionally and morphometrically at six locations (proximal, mid-, and distal locations of the two uterine horns) determined to be uniform and free of tissue bends and curves; epithelial height (mean μm ± SD) was measured from the basement membrane to the most apical cytoplasmic membrane.
5
Immunohistochemical Analysis of Testis Proteins
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Embedded testis tissue was dewaxed and rehydrated as previously described (Reid et al., 2012) and sections were subjected to antigen retrieval via immersion in 10 mM sodium citrate (pH 6) and microwaving at 1000 W for 10 min. Subsequent incubations were performed as previously described by Reid et al. (2012) with primary antibodies diluted 1:50 (ACE; PDIA6) or 1:100 (HSPA2) and incubated overnight at 48C. Following incubation in appropriate Alexa Fluor conjugated secondary antibodies (1:200), the slides were washed and mounted using a MOWIOL anti-fade reagent (13% Mowiol 4-88, 33% glycerol, 66 mM Tris, pH 8.5, 2.5% 1,4-diazabicyclo-[2,2,2] octane) and viewed with an Avio Imager A1 fluorescence microscope (Carl Zeiss Microimaging Inc., Thornwood, NY, USA) with images taken on an Olympus DP70 microscope camera.
6
Microscopic Observation of Starch Suspensions
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Observations of starch suspensions were carried out with an Olympus BX41 microscope (Olympus, Tokyo, Japan), using transmitted light, equipped with an Olympus DP70 microscope camera (Olympus, Tokyo, Japan).
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