The largest database of trusted experimental protocols

Deuterated standards

Manufactured by Merck Group
Sourced in United States

Deuterated standards are stable isotope-labeled compounds that are used as internal standards in analytical methods. They have the same chemical structure as the target analyte but with one or more hydrogen atoms replaced by deuterium, a heavier isotope of hydrogen. Deuterated standards are employed to improve the accuracy and precision of quantitative analyses, particularly in mass spectrometry applications.

Automatically generated - may contain errors

2 protocols using deuterated standards

1

Quantification of Phytochemicals in Endophytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Analytical-grade reagents were used to determine the POD, CAT, SOD, MDA, proline, and chlorophyll levels (Sangon Biotech [Shanghai] Co., Ltd. or Solarbio Science and Technology [Shanghai] Co., Ltd.). All deuterated standards and calibration samples for determining the ABA, auxin, GA1, GA3, and GA7 levels were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents for the high-performance liquid phase were sourced from Thermo Fisher Scientific Inc. Consumables such as petri dishes, agar, and sucrose for endophytic isolation and fermentation were purchased from Solarbio Science and Technology Co., Ltd. PDA, PDB, or water agar (WA) media were used. The PDB medium contained 200 g l−1 potato and 20 g l−1d-glucose. The PDA medium contained 200 g l−1 potato, 20 g l−1d-glucose and 15 g l−1 agar. The WA medium was prepared by adding 10 g agar to 1000 ml distilled water.
+ Open protocol
+ Expand
2

Antioxidant Compounds Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phenolic compounds standards were purchased from Extrasynthese (Genay, France) and Sigma-Aldrich (St. Louis, MO, USA). Carotenoid standards were purchased from Sigma-Aldrich. Deuterated standards were purchased from Sigma-Aldrich and Spectra2000. Solvents were purchased from AppliChem, Panreac Quimica SA (Barcelona, Spain), Sigma-Aldrich, and Trizol Reagent for RNA extraction was supplied by Fisher Scientific (Madrid, Spain) and the SYBR® Green assay for RT-PCR analysis by Bio-Rad Laboratories (Billerica, MA, USA) and primers were provided by IDT DNA Technologies (Leuven, Belgium). Ultra-pure water was produced by a Millipore system (Millipore, Bedford, MA, USA). More details are given in the Supplementary Materials S1.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!