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Blasticidin s

Manufactured by Carl Roth
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Sourced in Germany

Blasticidin S is a broad-spectrum antibiotic that inhibits protein synthesis in a wide range of organisms, including bacteria, fungi, and eukaryotic cells. It is commonly used as a selection marker in cell culture experiments.

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Lab products found in correlation

Puromycin, by Thermo Fisher Scientific (3 mentions) Doxycycline, by Merck Group (3 mentions) Bleomycin, by Merck Group (3 mentions) Ku60019, by Merck Group (3 mentions) 5 bromo 2 deoxyuridine b5002, by Merck Group (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Zeocin, by Thermo Fisher Scientific (1 mentions) Hygromycin b, by Thermo Fisher Scientific (1 mentions) Tetracycline, by Merck Group (1 mentions) Lentiviral particles, by AMS Biotechnology (1 mentions) Etoposide, by Merck Group (1 mentions) Cisplatin, by Merck Group (1 mentions) 5 bromo 2 deoxyuridine, by Merck Group (1 mentions)

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Blasticidin (6 citations)

5 protocols using blasticidin s

1

Assessing DNA Damage Response Pathways

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The following chemicals were purchased from Sigma-Aldrich: ATM inhibitor KU-60019 (SML1416-5MG), ATR inhibitor AZ-20 (SML1328-5MG), bleomycin (B8416), etoposide (E1383), doxycycline (D3072), cisplatin (P4394), 5-Bromo-2′-deoxyuridine (B5002). Puromycin was purchased from Thermo-Fisher (A1113802), blasticidin S was purchased from Carl Roth (CP14.2).
Constantin D, & Widmann C. (2020). ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells. Cell Death & Disease, 11(11), 1019.
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2

Transient and Stable Transfection of HeLa Cells

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HEK293 and HeLa cells were transfected with Lipofectamine 3000 (Life Technologies, L3000) according to the manufacturer’s instructions. mCherry tag containing vectors, CDK9-cGFP, CCNT1-cGFP and ZNF451-GFP vectors were transiently transfected in HeLa cells. NELFA-cGFP, NELFA-BG-nGFP, GFP-3FHBH (kind gift from Timmers lab, Freiburg and Akhtar Department, MPI Freiburg) were stably transfected in Flp-In T-Rex HeLa cells grown in 100 μg/mL Zeocin (Invitrogen, R25001) for at least three days before transfection. Stable positive clones were selected for at least three weeks in 15 μg/mL blasticidin S (Carl Roth CP14.1) and 100 μg/mL hygromycin B (Thermo Fisher Scientific, 10687010). Expression of proteins was induced with 1 μg/mL tetracycline (Sigma-Aldrich, T7660).
Rawat P., Boehning M., Hummel B., Aprile-Garcia F., Pandit A.S., Eisenhardt N., Khavaran A., Niskanen E., Vos S.M., Palvimo J.J., Pichler A., Cramer P, & Sawarkar R. (2021). Stress-induced nuclear condensation of NELF drives transcriptional downregulation. Molecular Cell, 81(5), 1013-1026.e11.
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3

Lentiviral-Mediated GFP Expression

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The introduction of GFP in patient-derived cell models was performed using lentiviral particles (Amsbio, Abingdon, UK, LVP436) following the manufacturer’s recommendations. Resistance to Blasticidin S (CarlRoth, Karlsruhe, Germany, #CP14.2) was used for selection, and GFP expression was monitored by immunofluorescence microscopy and flow cytometry.
Braun F.K., Rothhammer-Hampl T., Lorenz J., Pohl S., Menevse A.N., Vollmann-Zwerenz A., Bumes E., Büttner M., Zoubaa S., Proescholdt M., Schmidt N.O., Hau P., Beckhove P., Winner B, & Riemenschneider M.J. (2023). Scaffold-Based (Matrigel™) 3D Culture Technique of Glioblastoma Recovers a Patient-like Immunosuppressive Phenotype. Cells, 12(14), 1856.
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4

Assay Reagents for DNA Damage Response

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The following chemicals were purchased from Sigma-Aldrich: ATM inhibitor KU-60019 (SML1416-5MG), ATR inhibitor AZ-20 (SML1328-5MG), bleomycin (B8416), etoposide (E1383), doxycycline (D3072), cisplatin (P4394), 5-Bromo-2′-deoxyuridine (B5002. Puromycin was purchased from Thermo-Fisher (A1113802), blasticidin S was purchased from Carl Roth (CP14.2).
Constantin D., & Widmann C. (2020). ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells.
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5

Cellular Stress Response Assays

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The following chemicals were purchased from Sigma-Aldrich: ATM inhibitor KU-60019 (SML1416-5MG), ATR inhibitor AZ-20 (SML1328-5MG), bleomycin (B8416), etoposide (E1383), doxycycline (D3072), cisplatin (P4394), 5-Bromo-2′-deoxyuridine (B5002). Puromycin was purchased from Thermo-Fisher (A1113802), blasticidin S was purchased from Carl Roth (CP14.2).
Constantin D., & Widmann C. (2020). ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells. Cell Death and Disease, 11(11).
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