The FLAG-tagged RHOV eukaryotic expression vector was constructed using the PCR-amplified RHOV fragment and pcDNA3.0 (Invitrogen) with FLAG tag. The lung cancer cell lines A549 and H1299 were purchased from the American Type Culture Collection. The target sequence of RHOV shRNA was 5'-GGCTATTCTCAGTGCCATT-3'. Lentivirus was produced using pSIH-H1-Puro (System Biosciences) carrying RHOV shRNA according to the manufacturer's instructions, and was infected into A549 and H1299 lung cancer cells to generate individual clones. Anti-JNK, anti-phospho-JNK (pJNK), anti-c-Jun, anti-phospho-c-Jun and anti-RHOV were purchased from Proteintech; Anti-E-cadherin, anti-N-cadherin, anti-Snail and anti-Slug were purchased from BD Biosciences.
Anti slug
Manufactured by BD
Sourced in United States
Anti-Slug is a laboratory equipment designed to remove slugs and other small crawling organisms from surfaces. It utilizes a mechanical mechanism to capture and dispose of such organisms.
Automatically generated - may contain errors
Lab products found in correlation
Anti e cadherin, by BD (2 mentions)
Anti n cadherin, by BD (2 mentions)
Pcdna3, by Thermo Fisher Scientific (1 mentions)
Psih h1 puro, by System Biosciences (1 mentions)
Anti snail, by BD (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Anti jnk, by Proteintech (1 mentions)
Anti smad2 3, by Cell Signaling Technology (1 mentions)
Anti myc tag, by Cell Signaling Technology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti smad4, by Cell Signaling Technology (1 mentions)
Anti mdm2, by BD (1 mentions)
Anti snail, by R&D Systems (1 mentions)
Anti p smad2, by Cell Signaling Technology (1 mentions)
Anti p smad3, by Cell Signaling Technology (1 mentions)
Anti fibronectin, by BD (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
3 protocols using anti slug
1
Regulation of RHOV in Lung Cancer
2
Western Blot for Protein Expression Analysis
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Western blotting analysis of proteins in cell lysates was determined as previously described (Wu et al, 2014b (link)). Primary antibodies used were as follows: anti-MDM2 (Cat# 556353), anti-E-cadherin (Cat# 610405) and anti-Fibronectin (Cat# 610078) were purchased from BD (San Diego, CA, USA); anti-Slug (Cat# 9585), anti-Smad2/3 (Cat# 3102), anti-p-Smad2 (Cat# 3108), anti-p-Smad3 (Cat# 9520), anti-Smad4 (Cat# 9515) and anti-myc-tag (Cat# 2278) were from Cell Signaling Technology (Beverly, MA, USA); anti-Snail (Cat# AF3639) and anti-TGF-beta RI (Cat# AF3025) were from R&D; anti-β-Actin (Cat# sc-47778) was purchased from Santa Cruz (Santa Cruz, CA, USA).
3
Western Blot Antibody Panel for EMT Regulators
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The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920, monoclonal mouse, 1:1000, BD Biosciences), anti-Slug (#9585, monoclonal rabbit, 1:500, BD Biosciences), anti-CTCF (#07-729, polyclonal rabbit, 1:1000, Millipore Sigma), anti-BORIS/CTCFL (PA5-97639, polyclonal rabbit, 1:1000, ThermoFisher), anti-SMC3 (#5696, monoclonal rabbit, 1:1000, Cell Signaling), anti-YY1 (#46395S, monoclonal rabbit, 1:1000, Cell
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