Bortezomib was purchased from LC Laboratories (Woburn, MA, USA). For in vitro studies, the drug was reconstituted in dimethyl sulfoxide (DMSO) at a stock concentration of 1 mM and stored at −80°C. The small‐molecule inhibitors MKC3946 and GSK2606414 were bought from MedChemExpress (Monmouth Junction, NJ, USA). The stock solution for MKC3946 and GSK2606414 was prepared in DMSO at a concentration of 10 mM and stored at −20°C. The antibodies against p21Cip1, p27Kip1, X‐box‐binding protein 1 (Xbp1s), activating transcription factor 6 (Atf6), 78 kDa glucose‐regulated protein (Grp78), C/EBP homologous protein (Chop), cyclin D3, cyclin E1, cyclin‐dependent kinase 2 (CDK2), cyclin‐dependent kinase 4 (CDK4) and β‐actin were obtained from Proteintech (Wuhan, Hubei, China), and the antibody against activating transcription factor 4 (Atf4) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). All other chemicals were obtained from Sigma‐Aldrich (Burlington, MA, USA) unless otherwise specified.
Cyclin dependent kinase 4
Manufactured by Proteintech
Sourced in China, United States
Cyclin-dependent kinase 4 (CDK4) is a protein kinase that plays a crucial role in the regulation of the cell cycle. It is responsible for the phosphorylation of various substrates, including the retinoblastoma protein (Rb), which is a key regulator of the G1/S phase transition. CDK4 forms a complex with cyclin D, and this complex is essential for the progression of the cell cycle from the G1 phase to the S phase.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin e1, by Proteintech (1 mentions)
Cyclind3, by Proteintech (1 mentions)
Mkc3946, by MedChemExpress (1 mentions)
Gsk2606414, by MedChemExpress (1 mentions)
Cyclin dependent kinase 2, by Proteintech (1 mentions)
Bortezomib, by LC Laboratories (1 mentions)
β actin, by Proteintech (1 mentions)
Ripa buffer, by Sangon (1 mentions)
Cyclin d1, by Abcam (1 mentions)
2 protocols using cyclin dependent kinase 4
1
Bortezomib and Small-Molecule Inhibitors in Cell Studies
2
Mouse VSMC Protein Extraction and Analysis
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Mouse VSMCs were pulverized in RIPA buffer (Sangon Biotech Co., Ltd., Shanghai, China) and sonicated to disrupt the integrity of cell membranes and extract total proteins. Whole cell lysates were then centrifuged at 4000 × g for 15 min at 4°C. Total protein was quantified using the Bradford method. Total protein (20 µg per lane) was separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane. Other steps were performed as described previously (14 (link)). USP39 (cat. no. ab131244; 1:1,000; Abcam), cyclin D1 (cat. no. 60186-1-Ig; 1:1,000; ProteinTech Group, Inc., Chicago, IL, USA), and cyclin-dependent kinase 4 (CDK4; cat. no. 11026-1-AP; 1:1,000; ProteinTech Group, Inc.) antibodies were used as primary antibodies. β-tubulin antibody (cat. no. 10094-1-AP; ProteinTech Group, Inc.) was used to ensure equal loading quantities of protein samples.
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