Results were expressed as mean ± S.E. Tests of significance were carried out using the unpaired ANOVA with the Tukey Test embedded in the Origin 7.0 analysis software (OriginLab Corp., Northampton, MA). Data were considered statistically significant at *, p < 0.01, and **, p < 0.001.
Origin 7.0 analysis software
Manufactured by OriginLab
Sourced in United States
Origin 7.0 is a data analysis and graphing software. It provides tools for data manipulation, visualization, and statistical analysis. The software supports a wide range of data formats and offers features for curve fitting, peak analysis, and more.
Automatically generated - may contain errors
6 protocols using origin 7.0 analysis software
1
Comparative Assessment of Bioactive Compounds
2
Bioluminescence Resonance Energy Transfer Assay
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For BRET assays, raw data were imported in GraphPad Prism 9 software (San Diego, CA, USA), and each result is represented as mean ± S.E.M. of at least three independent experiments performed in triplicate. netBRET was calculated by subtracting background luminescence of cells only expressing Renilla Luciferase.
For electrophysiological assay, data analysis and offline leak subtraction were completed in Clampfit 10.4 (Axon Instruments), and all the analysis was performed using Origin 7.0 analysis software (OriginLab, Northampton, MA, USA).
Statistical analyses were performed using GraphPad Prism 9 and are described in the figure legend when applicable. A value was considered statistically significant when p < 0.05.
For electrophysiological assay, data analysis and offline leak subtraction were completed in Clampfit 10.4 (Axon Instruments), and all the analysis was performed using Origin 7.0 analysis software (OriginLab, Northampton, MA, USA).
Statistical analyses were performed using GraphPad Prism 9 and are described in the figure legend when applicable. A value was considered statistically significant when p < 0.05.
3
Neurotransmitter Receptor Pharmacology
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Data are reported as mean ± SEM. Two-way repeated measures-ANOVA was used to compare the groups and doses at the different times. The normality of data was analyzed by D’Agostino and Pearson tests. If the responses were measured only once, the differences were evaluated by one-way ANOVA followed by Tukey’s t test. P values less than 0.05 were considered significant. Statistical analysis was performed with Prism 8 (GraphPad). For electrophysiology, the data analysis was completed using Clampfit 10.4 (Axon Instruments), and all electrophysiology curves were fitted using Origin 7.0 analysis software (OriginLab).
4
Dose-Dependent Electrophysiological Analysis
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Data analysis and offline leak subtraction were completed in Clampfit 10.4 (Axon Instruments), and all electrophysiology curves were fitted using Origin 7.0 analysis software (OriginLab, Northampton, MA, USA). The menthol and cold dose-response relationships were fitted using the Hill equation. All averaged data are plotted as mean ± SEM and numbers in parentheses reflect the number of cells (n). The Shapiro-Wilk normality test was used for our two or multiple group comparison. Statistical analyses were completed with Origin 7.0 software using unpaired t-tests, or the nonparametric Mann-Whitney U test, when data were compared from two independent groups of cells. One-way analysis of variance (ANOVA) followed by the Bonferroni or Sidak’s post hoc test, or the nonparametric Kruskal-Wallis ANOVA test, was used for multiple comparisons, with the criterion for statistical significance set at p < 0.01.
5
Menthol and Cold Dose-Response Analysis
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Data analysis and o ine leak subtraction were completed in Clamp t 10.4 (Axon Instruments), and all electrophysiology curves were tted using Origin 7.0 analysis software (OriginLab, Northampton, MA, USA). The menthol and cold dose-response relationships were tted using the Hill equation. All averaged data are plotted as mean ± SEM and numbers in parentheses re ect the number of cells (n). The Shapiro-Wilk normality test was used for our two or multiple group comparison. Statistical analyses were completed with Origin 7.0 software using unpaired t-tests, or the nonparametric Mann-Whitney U test, when data were compared from two independent groups of cells. One-way analysis of variance (ANOVA) followed by the Bonferroni or Sidak's post hoc test, or the nonparametric Kruskal-Wallis ANOVA test, was used for multiple comparisons, with the criterion for statistical signi cance set at p < 0.01.
6
Menthol and Cold Dose-Response Analysis
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Data analysis and offline leak subtraction were completed in Clampfit 10.4 (Axon Instruments), and all electrophysiology curves were fitted using Origin 7.0 analysis software (OriginLab, Northampton, MA, USA). The menthol and cold dose-response relationships were fitted using the Hill equation. All averaged data are plotted as mean ± SEM and numbers in parentheses reflect the number of cells (n). Statistical analyses were completed with Origin 7.0 software using unpaired t-tests when data were compared from two independent groups of cells. One-way analysis of variance (ANOVA) followed by the Bonferroni or Sidak's post hoc test was used for multiple comparisons, with the criterion for statistical significance set at p < 0.01.
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