Segments were taken from the valve and flange of a Provox Vega voice prosthesis and mounted onto 12.5 mm aluminum SEM specimen stubs (Agar Scientific, AGG301) with superglue. The surfaces were imaged at ambient temperature with a Hitachi S-3400N scanning electron microscope, using the variable pressure scanning electron microscopy (VP-SEM) mode with a chamber pressure of 30 Pa and accelerating voltage of 10 kV. The backscattered electron (BSE) detector in conjunction with the energy dispersive X-ray spectroscopy (EDS) detector was used throughout with a working distance of 10 mm. The acquisition software was Oxford Instruments INCA and images were exported directly from this.
Agg301
Manufactured by Agar Scientific
Sourced in United Kingdom
The AGG301 is a laboratory instrument designed for the analysis of particle size distribution in liquid samples. It utilizes the principle of laser diffraction to measure the size of particles suspended in a liquid medium. The device accurately determines the particle size distribution, providing detailed information about the sample's characteristics.
Automatically generated - may contain errors
4 protocols using agg301
1
Scanning Electron Microscopy of Prosthetic Voice Device
2
Elemental Composition Analysis via EDX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Energy‐dispersive X‐ray spectroscopy (EDX) analyses were performed to confirm the elemental composition of the samples and to check for contamination after blasting. The specimens were mounted on aluminum stubs (12.5 mm ø, AGG301, Agar Scientific, UK) and fixed using carbon adhesive discs (12 mm ø, AGG3347N, Agar Scientific, UK). A narrow line of silver paint (G3691, Agar Scientific, UK) was used to enhance the conductivity of the specimens. The specimens were gold‐coated (approximately 5.0 nm) using the Q150T ES coater (Quorum Technologies, UK). The EDX spectra were generated using a LEO Ultra 55 scanning electron microscope at 10 kV (Carl Zeiss, Germany) equipped with an EDX detector (Inca, Oxford, UK).
3
Characterization of 3D-printed Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3D-printed samples were coated with an 8 nm gold layer using a SC 500A sputter (Emscope). After coating, samples were mounted on aluminium pint stubs (AGG301, Agar scientific) with carbon tabs. Sample imaging was carried out using a HITACHI SEM (FE SEM, JSM-6500F, JEOL and FE/VP SEM, TM3030Plus, HITACHI), spot size was set at 3.5 nm, and voltage was set at 10 kV. ImageJ software v. 1.48 from NIH (National Institutes of Health, USA) [21 (link)] was used to analyse the SEM micrographs.
4
Scanning Electron Microscopy of Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TCES and RES scaffolds were coated with a 10 nm gold layer (SC 500A, Emscope, Heathfield, UK) and mounted on aluminum pin stubs (AGG301, agar scientific) with carbon tabs (AGG3347N, Agar Scientific, Stansted, UK). Scaffolds were analyzed by scanning electron microscopy (SEM) FE SEM, JSM-6500F (JEOL, Tokyo, Japan) and FE/VP SEM, TM3030Plus (Hitachi, Tokyo, Japan ). Spot size and voltage were set up at 10 kV and 3.5 nm respectively for all samples. ImageJ software v. 1.48 from the National Institutes of Health (NIH, Bethesda, MD, USA) [42 (link)] was used to measure fiber diameter on the SEM micrographs. Three independent tests were performed for each material with 15 fibers measured for each micrograph (N = 3, n = 15).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!