Blood samples were diluted in PBS and PBMC fraction was isolated using SepMate 50 tubes (Stemcell Technologies) and human Lympholyte Separation Medium (Cedarlane). To ensure comparability with PBMC samples, a similar protocol for thymic leukocyte isolation was chosen. In brief, thymic tissue was placed on ice immediately after surgery and processed. A single-cell suspension was achieved by cutting the tissue using a scalpel and syringes. The cell suspension was washed and the leukocyte fraction isolated by density gradient centrifugation using human Lympholyte Separation Medium (Cedarlane). The resulting lymphocyte fraction was washed, cryopreserved in 10% DMSO in fetal calf serum (FCS; Biochrom) and stored in the vapor phase of a liquid nitrogen tank until further analysis.
Human lympholyte separation medium
Manufactured by Cedarlane
Sourced in Canada
Human Lympholyte Separation Medium is a laboratory product used for the isolation and separation of human lymphocytes from whole blood samples. It facilitates the density-based separation of mononuclear cells from other blood components. The product enables the isolation of viable and functional lymphocytes for further analysis and research applications.
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Lab products found in correlation
3 protocols using human lympholyte separation medium
1
Isolation of PBMC and Thymic Leukocytes
2
Isolation of PBMC and Thymic Leukocytes
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Blood samples were diluted in PBS and PBMC fraction was isolated using SepMate 50 tubes (Stemcell Technologies) and human Lympholyte Separation Medium (Cedarlane). To ensure comparability with PBMC samples, a similar protocol for thymic leukocyte isolation was chosen. In brief, thymic tissue was placed on ice immediately after surgery and processed. A single-cell suspension was achieved by cutting the tissue using a scalpel and syringes. The cell suspension was washed and the leukocyte fraction isolated by density gradient centrifugation using human Lympholyte Separation Medium (Cedarlane). The resulting lymphocyte fraction was washed, cryopreserved in 10% DMSO in fetal calf serum (FCS; Biochrom) and stored in the vapor phase of a liquid nitrogen tank until further analysis.
3
Autoimmune Myasthenia Gravis Immunobiology
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Peripheral blood and serum samples were obtained from 38 patients with AChR MG and 21 age- and sex-matched healthy donors at the Neuromuscular Center, University Hospital Zurich, Switzerland. Patients with AChR MG showed typical clinical and serologic features (Ingelfinger et al., eTable 1, links.lww.com/NXI/A800 ) and were not treated with immunosuppressive medication including steroids at the time of the blood draw. The CARE Reporting Guidelines were used.20 (link) Peripheral blood mononuclear cells (PBMCs) were isolated using SepMate 50 tubes (STEMCELL Technologies, Vancouver, Canada) and human Lympholyte Separation Medium (Cedarlane, Burlington, Ontario) and stored in liquid nitrogen until use.
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