Western blot analysis was conducted as introduced previously [26] , using the following antibodies: anti-TRPC5OS (Quan-biotech, Wuhan, China), anti-ENO1 (Abcam, Cambridge, MA, USA), anti-PI3K (Abcam), anti-pPI3K (Abcam), anti-Akt (Abcam), anti-phospho-Akt (Abcam), and anti-β-actin (Cell Signaling, Danvers, MA, USA). β-Actin was used as an internal control. Peroxidase-conjugated Affinipure goat anti-mouse and anti-rabbit IgG (H+L,Jackson Immunoresearch, City, PA, USA) were utilized as secondary antibodies.
Peroxidase conjugated affinipure goat anti mouse and anti rabbit igg h l
Manufactured by Jackson ImmunoResearch
Sourced in United States
Peroxidase-conjugated Affinipure goat anti-mouse and anti-rabbit IgG (H+L) is a secondary antibody that binds to the heavy and light chains of mouse and rabbit immunoglobulin G (IgG) and is conjugated to the enzyme horseradish peroxidase (HRP).
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho akt, by Abcam (1 mentions)
Anti eno1, by Abcam (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti p pi3k, by Abcam (1 mentions)
Anti akt, by Abcam (1 mentions)
Anti pi3k, by Abcam (1 mentions)
Anti zfp57, by Abcam (1 mentions)
Anti cyclin d1, by Abcam (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Anti c myc, by Abcam (1 mentions)
Anti α tubulin, by Abcam (1 mentions)
2 protocols using peroxidase conjugated affinipure goat anti mouse and anti rabbit igg h l
1
Western Blot Analysis of Protein Targets
2
Western Blot Analysis of Epigenetic Regulators
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Cells were harvested for Western blot analysis as described29 (link).
The anti-ZFP57 (abcam, Cambridge, UK, 1:1000), the anti-MEST (abcam, 1:2000), the anti-β-catenin (abcam, 1:5000), the anti-cyclin D1 (abcam, 1:5000), the anti-c-Myc (abcam, 1:5000), the anti-GAPDH (CellSignaling, Danvers, MA, 1:1000) and the anti-α-Tubulin (abcam, 1:5000) were used as the primary antibodies. GAPDH and α-Tubulin were used as an internal control. A 1:5000–10,000 dilution of the Peroxidase-conjugated Affinipure GOAT anti-Mouse and anti-Rabbit IgG (H + L) (Jackson Immunoresearch, USA) was used as the secondary antibody.
The anti-ZFP57 (abcam, Cambridge, UK, 1:1000), the anti-MEST (abcam, 1:2000), the anti-β-catenin (abcam, 1:5000), the anti-cyclin D1 (abcam, 1:5000), the anti-c-Myc (abcam, 1:5000), the anti-GAPDH (CellSignaling, Danvers, MA, 1:1000) and the anti-α-Tubulin (abcam, 1:5000) were used as the primary antibodies. GAPDH and α-Tubulin were used as an internal control. A 1:5000–10,000 dilution of the Peroxidase-conjugated Affinipure GOAT anti-Mouse and anti-Rabbit IgG (H + L) (Jackson Immunoresearch, USA) was used as the secondary antibody.
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