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Clec9a

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CLEC9A is a cell surface receptor protein that is expressed on a subset of dendritic cells. It functions in the recognition and uptake of dead or dying cells.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Cd209, by BioLegend (1 mentions) Clec10a, by BioLegend (1 mentions) Cd207, by BioLegend (1 mentions) Cd303, by Miltenyi Biotec (1 mentions) Lsr fortessa cytometer, by BD (1 mentions) Cellquest, by BD (1 mentions) Facscalibur flow, by BD (1 mentions) Pd l1, by BD (1 mentions) Bdca3, by Miltenyi Biotec (1 mentions) Kaluza, by Beckman Coulter (1 mentions) Cd103, by BioLegend (1 mentions)

2 protocols using clec9a

1

Characterization of Dendritic Cell Subsets

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The purity of MDDCs and mDCs were verified by flow cytometry, and was shown to be ~90% pure for CD11c and CD1c respectively. The DCs were stained for C-type lectins using anti-CD205, -CD206, -CD207, -CD209, -CLEC4A, -CLEC9A, -CLEC10A, -CLEC12A (Biolegend) and -CD303 (Miltenyi Biotec) antibodies conjugated to PE. FACS data was acquired on BD FACS Calibur (BD Biosciences, San Jose, CA, USA) and analyzed with FlowJo software (Tree Star).
Sagar D., Singh N.P., Ginwala R., Huang X., Philip R., Nagarkatti M., Nagarkatti P., Neumann K., Ruland J., Andrews A.M., Ramirez S.H., Khan Z.K, & Jain P. (2017). Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity. Scientific Reports, 7, 2707.
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2

Phenotypic Analysis of Dendritic Cells

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DC subsets from PBMC or SLN single-cell suspensions, isolated and prepared as previously described,16 17 (link) were phenotypically analyzed by 4 or 10 color flow cytometry, with the following mAbs, which were diluted in Phosphate Buffered Saine (PBS) supplemented with 0.1% Bovine Serum Albumin (BSA) and 0.02% NaN3, and incubated for 30 min at 4°C: CD11c, CD1a, CD14, PD-L1, CD16 (BD Biosciences), CD40, CD83 (Beckman Coulter), CD86, CD80 (BD Pharmingen), BDCA3 (Miltenyi Biotec), CLEC9A, CD1c, BDCA3, CD103 (Biolegend), CD83, PD-L1 (BD Horizon). After incubation, cells were washed in FACS buffer to remove excess antibodies and used for flow cytometric analyses. Flow cytometric analyses were performed on a FACS-Calibur flow or LSR Fortessa cytometer (Becton Dickinson), equipped with CellQuest or FACSDiva data acquisition software, respectively; data were analyzed using CellQuest (BD Biosciences) or Kaluza (Beckman Coulter) analysis software.
Koster B.D., López González M., van den Hout M.F., Turksma A.W., Sluijter B.J., Molenkamp B.G., van Leeuwen P.A., Vosslamber S., Scheper R.J., van den Eertwegh A.J., van den Tol M.P., Jordanova E.J, & de Gruijl T.D. (2021). T cell infiltration on local CpG-B delivery in early-stage melanoma is predominantly related to CLEC9A+CD141+ cDC1 and CD14+ antigen-presenting cell recruitment. Journal for Immunotherapy of Cancer, 9(3), e001962.
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