The pneumococcal R6 wild-type and ΔPn-aqpC strains were grown in BHI broth until the early stationary-phase, and then the cultures were centrifuged at 12,000 rpm for 15 min. The supernatants were filtered using a 0.22 μm disposable needle filter (Millipore, Billerica, MA).Then, the formate, acetate, and lactate concentrations in the supernatants were measured using HPLC (Thermo Fisher Scientific). The chemicals formate, acetate, and lactate at known concentrations were included and the concentrations of corresponding chemicals in the tested samples were calculated according to chemical standards.
0.22 μm disposable needle filter
Manufactured by Merck Group
The 0.22 μm disposable needle filter is a laboratory equipment product designed to remove particulates and sterilize fluids. It features a 0.22 micron pore size membrane that effectively filters out microorganisms and other contaminants from liquids. The filter is intended for single-use and is constructed with inert materials for safe and reliable filtration.
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Lab products found in correlation
2 protocols using 0.22 μm disposable needle filter
1
Measuring Metabolite Levels in Pneumococcal Strains
2
Measuring Metabolite Levels in Pneumococcal Strains
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The pneumococcal R6 wild-type and ΔPn-aqpC strains were grown in BHI broth until the early stationary-phase, and then the cultures were centrifuged at 12,000 rpm for 15 min. The supernatants were filtered using a 0.22 μm disposable needle filter (Millipore, Billerica, MA).Then, the formate, acetate, and lactate concentrations in the supernatants were measured using HPLC (Thermo Fisher Scientific). The chemicals formate, acetate, and lactate at known concentrations were included and the concentrations of corresponding chemicals in the tested samples were calculated according to chemical standards.
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